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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Growth Differentiation Factor 15 (GDF15)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPC034Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 14.0kDa
Predicted isoelectric point 7.7
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
生长分化因子15(GDF15)重组蛋白
Subcellular Location Secreted
Residues Ala195~Ile308 (Accession # Q99988) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-ARARNG DHCPLGPGRC CRLHTVRASL EDLGWADWVL SPREVQVTMC IGACPSQFRA ANMHAQIKTS LHRLKPDTVP APCCVPASYN PMVLIQKTDT GVSLQTYDDL LAKDCHCI
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
生长分化因子15(GDF15)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验1 月内连登 4 顶刊,这个炙手可热的明星蛋白 GDF15 到底什么来路?
作用。图片来源:Cell Metabolism这是 GDF15 继 2019 年 12 月先后登上 Nature Metabolism 和 Nature 之后,再次出现在顶级期刊上。下面就随着小编来看看 GDF15 这个热门蛋白的来路以及相关的 4 篇论文吧。GDF15 有啥来路?生长分化因子-15(Growth/differentiation factor 15,GDF15)是转化生长因子-β 超家族(TGFβ super family)的一员。此前 GDF15 被发现在创伤过后的多种器官出现明显
重组菌的生长温度,降低培养温度是减少包涵体形成的最常用的方法,较低的生长温度降低了无活性聚集体形成的速率和疏水相互作用,从而可减少包涵体的形成[4]。 2、 添加可促进重组蛋白质可溶性表达的生长添加剂,培养E.coli时添加高浓度的多醇类、蔗糖或非代谢糖可以阻止分泌到周质的蛋白质聚集反应,在最适浓度范围内添加这些添加剂不会影响细胞的生长、蛋白质的合成或运输,其它促重组蛋白质可溶性表达的生长添加剂还有乙醇(诱导热休克蛋白的表达)、低分子量的巯基或二硫化合物(影响细胞周质的还原态,从而影响
三句话读懂一篇 CNS:二甲双胍减轻体重的原因找到了;限时禁食让人长寿,却影响生育能力
器可以降低患痴呆症的风险,高达 8.2% 的痴呆症病例可以通过预防听力损失来预防。 图 9:来源 Lancet Public Health 10. Cell Metabolism:二甲双胍降低食欲、减轻体重的作用机理 二甲双胍一直被誉为是神药般的存在,对减肥亦有作用。 2023 年 4 月 14 日,多伦多综合医院研究所 Tony K.T. Lam 团队在 Cell Metabolism 杂志发表研究论文 Metformin triggers a kidney GDF15-dependent
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