Calreticulin (CALR) (Middle Re

Calreticulin (CALR) (Middle Region) 抗体抗原
Calreticulin (CALR)
别名
crc, crt, zgc:85636, CG9429, Clr, Dmel\\CG9429, anon-EST:Liang-2.11, anon-EST:Liang-2.17, anon-EST:fe3D7, clone 2.11, clone 2.17, eth, potp, cc1qr, ssa, NV19007, CRT, RO, SSA, cC1qR, Calregulin, CRP55 ... show more
抗原表位
其他选择 Middle Region
适用
其他选择 人, 小鼠
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 非结合性
应用范围
其他选择 酵素免疫分析法（EIA）, Western Blotting (WB)
Pubmed 找到1个引用
规格 0.4 mL
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb452803
产品细节 Calreticulin (CALR) (Middle Region) 抗体
免疫原 KLH conjugated synthetic peptide selected from the Center region of human CALR
特异性 This antibody detects Calreticulin at Center.
交叉反应 （详细） Species reactivity (tested):Human, Mouse.
纯化方法 Purified through a protein A column, followed by peptide affinity purification
目标详细情况 Calreticulin (CALR) (Middle Region) 抗体
别名 Calreticulin
背景 Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.Synonyms: CALR, CRP55, CRTC, Calregulin, ERp60, HACBP, grp60
分子量 48142 Da
基因ID 811
NCBI登录号 NP_004334
UniProt P27797
研究领域 Signaling, Organelles, Autophagy
使用细节 Calreticulin (CALR) (Middle Region) 抗体
应用备注 Western blot: 1: 100 - 1: 500. ELISA: 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理 Calreticulin (CALR) (Middle Region) 抗体
状态 Liquid
浓度 0.25 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.