- ￥495 - 4772
- Invent
- 美国
- SN-006
- 2026年04月26日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
4℃
- 英文名：
Minute™ Detergent-Free Total Protein Extraction Kit for Animal Cells/Tissues
- 规格：
50 Preps/4 Preps
| 规格： | 50 Preps | 产品价格： | ￥4772.0 |
|---|---|---|---|
| 规格： | 4 Preps | 产品价格： | ￥495.0 |
MinuteTM 无表面活性剂的动物细胞/组织总蛋白提取试剂盒
目录号：SN-006
描述
无表面活性剂的动物细胞/组织总蛋白提取试剂盒，由优化细胞裂解液和0.6 ml离心管柱组成，能够迅速从动物细胞（昆虫/哺乳动物等）和组织（无脊椎动物和脊椎动物）中提取无表面活性剂的总蛋白。试剂盒中的裂解液不含任何表面活性剂和EDTA。离心管柱技术提取蛋白质，样品量最小可处理20ul，最大可达500ul。从细胞/组织中提取总蛋白质操作时间小于5分钟，得率可达1-5mg/ml。
应用
提取的蛋白质样品可以应用于蛋白质组学（液相色谱/质谱），IP，ELISA，2D分析，等电聚焦，SDS-PAGE, immunoblottings等。
Figure 1. Comparison of protein profiles of different animal tissues extracted by denaturing lysis buffer and detergent-free extraction buffer. Lane 1, Drosophila cultured cells (SL2); Lane 2, Drosophila adult flies; Lane 3, gold fish muscle; Lane 4, mouse liver. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.
操作步骤
SN-006
操作流程图
Protocol
请点击产品资料下载查看
产品特点
速度快，操作简单
小样本量：最小可处理20ul
无蛋白丢失，高得率
无需超声处理，煮沸，或反复冻融
不含任何表面活性剂和EDTA
参考文献
1. Seo, J-H. et al (2014). Directing stem cell differenciation by changing the molecular mobility of supramolecular surfaces. Advanced Healthcare Materials. DOI: 10.1002/adhm.201400173
2. M. Takada, S. Chiba et al. (2015). Inflammatory responses to neutral fatand fatty acids in multiple organs in a rat model of fat embolism syndrome, Forensic Science International . DOI: org/10.1016/j.forsciint.2015.07.011
3. Seo J. et al. (2016).Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes. RSC Advances. DOI: 10.1039/C6RA03967G
4. Xu, Wenteng, et al. (2016).Ubiquitin ligase gene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination. Gene (2016), DOI: 10.1016/j.gene.2016.07.062
5.Yamamoto, H., Hayano, S., Okuno, Y., Onoda, A., Kato, K., Nagai, N., ... & Kato, T. (2020). Phosphorylated proteome analysis of a novel germline ABL1 mutation causing an autosomal dominant syndrome with ventricular septal defect. International Journal of Cardiology.
6.Pietras, P., Leśniczak-Staszak, M., Kasprzak, A., Andrzejewska, M., Jopek, K., Sowiński, M., ... & Szaflarski, W. (2021). MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells. International Journal of Molecular Sciences, 22(22), 12121.
7.Minerva, D., Othman, N. L., Nakazawa, T., Ito, Y., Yoshida, M., Goto, A., & Suzuki, T. (2022). A New Chemotactic Mechanism Governs Long-Range Angiogenesis Induced by Patching an Arterial Graft into a Vein. International Journal of Molecular Sciences, 23(19), 11208.
8.Tatsuya Yoshida; Satoya Yoshida; Kohei Inukai; Katsuhiro Kato; Yoshimitsu Yura; et al(2024). ALPK2 prevents cardiac diastolic dysfunction in heart failure with preserved ejection fraction. The FASEB Journal.DOI 10.1096/fj.202402103r
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- 作者
- 内容
- 询问日期
文献和实验1. Seo, J-H. et al (2014). Directing stem cell differenciation by changing the molecular mobility of supramolecular surfaces. Advanced Healthcare Materials. DOI: 10.1002/adhm.201400173
2. M. Takada, S. Chiba et al. (2015). Inflammatory responses to neutral fatand fatty acids in multiple organs in a rat model of fat embolism syndrome, Forensic Science International . DOI: org/10.1016/j.forsciint.2015.07.011
3. Seo J. et al. (2016).Dynamic polyrotaxane-coated surface for effective differentiation of mouse induced pluripotent stem cells into cardiomyocytes. RSC Advances. DOI: 10.1039/C6RA03967G
4. Xu, Wenteng, et al. (2016).Ubiquitin ligase gene neurl3 plays a role in spermatogenesis of half-smooth tongue sole (Cynoglossus semilaevis) by regulating testis protein ubiquitination. Gene (2016), DOI: 10.1016/j.gene.2016.07.062
5.Yamamoto, H., Hayano, S., Okuno, Y., Onoda, A., Kato, K., Nagai, N., ... & Kato, T. (2020). Phosphorylated proteome analysis of a novel germline ABL1 mutation causing an autosomal dominant syndrome with ventricular septal defect. International Journal of Cardiology.
6.Pietras, P., Leśniczak-Staszak, M., Kasprzak, A., Andrzejewska, M., Jopek, K., Sowiński, M., ... & Szaflarski, W. (2021). MVP Expression Facilitates Tumor Cell Proliferation and Migration Supporting the Metastasis of Colorectal Cancer Cells. International Journal of Molecular Sciences, 22(22), 12121.
7.Minerva, D., Othman, N. L., Nakazawa, T., Ito, Y., Yoshida, M., Goto, A., & Suzuki, T. (2022). A New Chemotactic Mechanism Governs Long-Range Angiogenesis Induced by Patching an Arterial Graft into a Vein. International Journal of Molecular Sciences, 23(19), 11208.
8.Tatsuya Yoshida; Satoya Yoshida; Kohei Inukai; Katsuhiro Kato; Yoshimitsu Yura; et al(2024). ALPK2 prevents cardiac diastolic dysfunction in heart failure with preserved ejection fraction. The FASEB Journal.DOI 10.1096/fj.202402103r
9. Marta Leśniczak-Staszak; Paulina Pietras; Marcin Ruciński; Ryan Johnston; Mateusz Sowiński; et al.(2024). Stress granule-mediated sequestration of EGR1 mRNAs correlates with lomustine-induced cell death prevention.Journal of Cell Science.DOI 10.1242/jcs.261825
10.Leyi Liao; Ziying Liu; Lei Liu; Can Huang; Yiyi Li; et al.(2025).Targeting the ceramidase ACER3 attenuates cholestasis in mice by mitigating bile acid overload via unsaturated ceramide-mediated LXRβ signaling transduction.Nature Communications.DOI 10.1038/s41467-025-57330-7
11.Kai Wang; Leyi Liao; Hanbiao Liang; Pengxiang Huang; Qingping Li; et al.(2025).Ceramide(d18:1/18:1)-NDUFA6 interaction inactivates respiratory complex I to attenuate oxidative-stress-driven pathogenesis in liver ischemia/reperfusion injury.JCI Insight.DOI 10.1172/jci.insight.187083
12.Zhenzhen He; Dongxue Wang; Jiahao Chen; Xin Hu; Danmeng Shuai.(2025).Peroxide Disinfection of Vesicle-Cloaked Murine Norovirus Clusters: Vesicle Membranes Protect Viruses from Inactivation.Environmental Science & Technology.DOI:10.1021/acs.est.4c13695
使用Lipofectamine™2000转染Stealth™ RNA或者siRNA进入哺乳动物细胞
Lipofectamine™ 2000试剂（使用前贮存在+4℃） ・Opti-MEM® I 低血清培养基（使用前37℃预热） ・合适的组织培养板及其它 转染步骤 使用Lipofectamine™ 2000转染Stealth™ RNA或者siRNA进入哺乳动物细胞时，使用下述步骤。参阅下列表格《推荐试剂的量和体积》以确定加入不同组织培养方式的试剂量以及体积。使用推荐Lipofectamine™ 2000的量
如果您的样本是组织，取样的过程非常重要。组织内部是有内源性 RNase 存在的，组织离体后内源性 RNase 则开始发挥作用，RNA 产生降解。所以离体的组织必须立马放在液氮中速冻，之后转移至 -80℃ 长期保存，且避免反复冻融。研磨组织的过程中需要保证在液氮环境中研磨。RNA 提取的过程中，保证样本的上样量不超过提取试剂盒说明书中规定的最大上样量，上样量过多，同样会造成 RNA 降解。 ②如果您的样本是细胞，则样本搜集过程要简单的多了，只要保证细胞状态良好（细胞状态差也容易造成提取的 RNA 降解
原理将样品经增菌后划平板分离单菌落，挑取可疑菌落到胰蛋白胨葡萄糖酵母浸膏肉汤 (TPGY)培养基培养，对培养物用 DNA 提取试剂盒抽提 DNA，进行 PCR 扩增，用琼脂糖凝胶电泳检验 PCR 产物中是否含有肉毒杆菌的的特征条带，从而初步判断食品是否被肉毒杆菌污染。 二、仪器和试剂 紫外检测仪 NanoDrop1000 (Eppendorf)，台式微量冷冻离心机 Microfuge 22R（Beckman Counter），凝胶成像分析系统 Gel DocTM XR （Bio
