SD-008/SN-009 Minute™ 总蛋白提取试剂盒

Minute^TM 植物组织总蛋白提取试剂盒

目录号：SD-008/SN-009

描述    

植物组织总蛋白提取试剂盒，由优化细胞裂解液和0.6 ml离心管柱组成，能够迅速从植物组织（叶片，种子，柔软的干和根等）中提取总蛋白。试剂盒同时提供天然和变性两种不同的裂解液，可供用户选择。离心管柱技术提取蛋白质，可从20-200mg植物组织中中提取总蛋白质，操作时间5-8分钟，得率可达2-8mg/ml。

应用    

提取的蛋白质样品可以应用于SDS-PAGE, immunoblottings, ELISA, IP, ELISA，蛋白质分析和小规模蛋白层析纯化。

Figure 1. Plant protein profiles extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, snow pea; lane 2, corn kernel; 3, broccoli leaves; 4, spinach leaves and 5, chili pepper leaves.

操作步骤

操作流程图

  Protocol    

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参考文献  

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