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Minute™ Total Protein Extraction Kit for Plant Tissues
50 Preps/4 Preps
规格: | 50 Preps | 产品价格: | ¥3577.0 |
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规格: | 4 Preps | 产品价格: | ¥374.0 |
MinuteTM 植物组织总蛋白提取试剂盒
目录号:SD-008/SN-009
描述
植物组织总蛋白提取试剂盒,由优化细胞裂解液和0.6 ml离心管柱组成,能够迅速从植物组织(叶片,种子,柔软的干和根等)中提取总蛋白。试剂盒同时提供天然和变性两种不同的裂解液,可供用户选择。离心管柱技术提取蛋白质,可从20-200mg植物组织中中提取总蛋白质,操作时间5-8分钟,得率可达2-8mg/ml。
应用
提取的蛋白质样品可以应用于SDS-PAGE, immunoblottings, ELISA, IP, ELISA,蛋白质分析和小规模蛋白层析纯化。
Figure 1. Plant protein profiles extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue. Lane 1, snow pea; lane 2, corn kernel; 3, broccoli leaves; 4, spinach leaves and 5, chili pepper leaves.
操作步骤
操作流程图
Protocol请点击产品资料下载查看
参考文献20. Wang chengliang et al(2020).Chloroplastic Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice.NEW PHYTOLOGIST
21. Chen, J., Huang, H., Wei, S., Cui, H., Li, X., & Zhang, C. (2020). Glyphosate resistance in Eleusine indica: EPSPS overexpression and P106A mutation evolved in the same individuals. Pesticide Biochemistry and Physiology.
22. Lang, H., He, Y., Sun, J., Li, F., & Ma, D. Integrative hormone and transcriptome analyses underline the role of abscisic acid in seed shattering of weedy rice.
23.Li, P., Ma, J., Sun, X., Zhao, C., Ma, C., & Wang, X. RAB GTPASE HOMOLOG 8D is required for maintenance of both the root stem cell niche and meristem. The Plant Journal.
24.Zhen, X., Zheng, N., Yu, J., Bi, C., & Xu, F. (2021). Autophagy mediates grain yield and nitrogen stress resistance by modulating nitrogen remobilization in rice. PloS one, 16(1), e0244996.
25.Li, K., Zhou, X., Sun, X., Li, G., Hou, L., Zhao, S., ... & Wang, X. (2021). Coordination between MIDASIN 1-mediated ribosome biogenesis and auxin for modulating plant development. Journal of Experimental Botany.
26.Subramanyam, S., Nemacheck, J. A., Bernal-Crespo, V., & Sardesai, N. (2021). Insect derived extra oral GH32 plays a role in susceptibility of wheat to Hessian fly. Scientific Reports, 11(1), 1-16.
27.Cheng, Z., Dong, S., Li, Z., Zou, L., Zhao, P., Guo, X., ... & Peng, M. (2021). Global identification of full-length cassava lncRNAs unveils the role of CRIR1 in cold stress response. Authorea Preprints.
28.Guojiao Wang, Faliang Zeng, Peng Song, Bei Sun, Qi Wang, Jiayu Wang.(2022)Effects of reduced chlorophyll content on photosystem functions and photosynthetic electron transport rate in rice leaves.Journal of Plant Physiology.
29.Villegas-Estrada, B., Sánchez, M. A., & Valencia-Jiménez, A. (2022). Foliar Infiltration of Virus-Derived Small Hairpin RNAs Triggers the RNAi Mechanism against the Cucumber Mosaic Virus. International Journal of Molecular Sciences, 23(9), 4938.
30.Tal, L., Palayam, M., Ron, M. et al. A conformational switch in the SCF-D3/MAX2 ubiquitin ligase facilitates strigolactone signalling. Nat. Plants (2022). https://doi.org/10.1038/s41477-022-01145-7
31.DONG, S. M., Liang, X. I. A. O., LI, Z. B., Jie, S. H. E. N., YAN, H. B., LI, S. X., ... & Ming, P. E. N. G. (2022). A novel long non-coding RNA, DIR, increases drought tolerance in cassava by modifying stress-related gene expression1. Journal of Integrative Agriculture.
32.Tal, L., Guercio, A. M., Varshney, K., Young, A., Gutjahr, C., & Shabek, N. (2023). C-terminal conformational changes in SCF-D3/MAX2 ubiquitin ligase are required for KAI2-mediated signaling. bioRxiv, 2023-01.
33.Chen, J., Li, Z., Cui, H., Yu, H., & Li, X. (2023). Gene Amplification of EPSPS with a Mutation in Conserved Region: The Evolved Glyphosate Resistance Mechanism in Eleusine indica. Agronomy, 13(3), 699.
34.Zhou, Y., Jin, W., Duan, M., She, X., Zhu, S., Zhou, X., ... & Zhu, D. (2023). Effects of exogenous strain fermentation on protein structure and allergenicity of Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.). Food Bioscience, 102541.
35.Zhou, Y., Yu, S., She, X., & Zhou, X. (2023). Study on the reduction of Tartary buckwheat allergenicity during Pediococcus pentosaceus fermentation by HPLC-MS/MS analysis. Food Chemistry: X, 100773.
36.Ganapathy, J., Hand, K. A., & Shabek, N. (2023). Analysis of 26S Proteasome Activity Across Arabidopsis Tissues. bioRxiv, 2023-09.
37.Bartos, A., Majak, I., & Leszczyńska, J. (2023). Detection of Bet v 1 Homologous Proteins and Plant Profilins by Indirect ELISA. In Food Allergens: Methods and Protocols (pp. 305-310). New York, NY: Springer US.
38.Jing W, Gong F, Liu G, Deng Y, Liu J, Yang W, Sun X, Li Y, Gao J, Zhou X, Ma N. Petal size is controlled by the MYB73/TPL/HDA19-miR159-CKX6 module regulating cytokinin catabolism in Rosa hybrida. Nat Commun. 2023 Nov 4;14(1):7106. doi: 10.1038/s41467-023-42914-y. PMID: 37925502.
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资料下载:
8.SD-008 SN-009-中文说明书 v5.pdf 附件下载 (下载 1 次)
8-SD-008-0SN-009_v5.pdf 附件下载 (下载 0 次)
008 009.jpg 附件下载 (下载 2 次)
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8.SD-008 SN-009-中文说明书 v5.pdf 附 (下载 1 次)
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008 009.jpg 附 (下载 2 次)