- 询价
- 沪震生物
- 进口/国产
- hz972Hu01
- 2025年07月14日
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- 详细信息
- 询价记录
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- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Pigment Epithelium Derived Factor (PEDF)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPB972Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 47.8kDa
Predicted isoelectric point 6.3
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
色素上皮衍生因子(PEDF)重组蛋白
Subcellular Location Secreted. Melanosome
Residues Asn21~Pro415 (Accession # P36955) with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NPASPPEEGS PDPDSTGALV EEEDPFFKVP VNKLAAAVSN FGYDLYRVRS STSPTTNVLL SPLSVATALS ALSLGAEQRT ESIIHRALYY DLISSPDIHG TYKELLDTVT APQKNLKSAS RIVFEKKLRI KSSFVAPLEK SYGTRPRVLT GNPRLDLQEI NNWVQAQMKG KLARSTKEIP DEISILLLGV AHFKGQWVTK FDSRKTSLED FYLDEERTVR VPMMSDPKAV LRYGLDSDLS CKIAQLPLTG SMSIIFFLPL KVTQNLTLIE ESLTSEFIHD IDRELKTVQA VLTVPKLKLS YEGEVTKSLQ EMKLQSLFDS PDFSKITGKP IKLTQVEHRA GFEWNEDGAG TTPSPGLQPA HLTFPLDYHL NQPFIFVLRD TDTGALLFIG KILDP
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
色素上皮衍生因子(PEDF)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 色素上皮细胞衍生因子 ( PEDF )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和唾液等其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 PEDF 单抗包被于酶标板上，标准品和样品中的 PEDF与单抗结合，加入生物素化的抗人
小鼠色素上皮细胞衍生因子(PEDF)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠 色素上皮细胞衍生因子 ( PEDF )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和唾液等其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 PEDF 单抗包被于酶标板上，标准品和样品中的 PEDF与单抗结合，加入生物
在 Am J Physiol Cell Physiol 上的一篇研究论文。题目：High glucose promotes the migration of retinal pigment epithelial cellsthrough increased oxidative stress and PEDF expression。从题目中，我们就可以看出，本文主要讲的就是高糖通过增加氧化应激和 PEDF 表达促进视网膜色素上皮细胞的迁移。题目有点长，其实文章内容也很多，多达 17 页！对于初学
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