- 询价
- 沪震生物
- 进口/国产
- hz912Hu01
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Defensin Alpha 5, Paneth Cell Specific (DEFa5)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPB912Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 13.8kDa
Predicted isoelectric point 5.9
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
防御素α5(DEFα5)重组蛋白
Subcellular Location Secreted. Cytoplasmic vesicle, secretory vesicle.
Residues Glu20~Arg94 (Accession # Q01523) with two N-terminal Tags, His-tag and S-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5%
trehalose, 0.01% sarcosyl and preservative.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is
listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- E SLQERADEAT TQKQSGEDNQ
DLAISFAGNG LSALRTSGSQ ARATCYCRTG RCATRESLSG VCEISGRLYR LCCR
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store
at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein.
The loss rate was determined by accelerated thermal degradation test, that is, incubate the
protein at 37oC for 48h, and no obvious degradation and precipitation were observed.
(Referring from China Biological Products Standard, which was calculated by the Arrhenius
equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make
location and size approximation of proteins of interest quick and easy.
防御素α5(DEFα5)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验试剂盒使用说明： 检测范围： 96T 20pg/ml-480pg/ml 使用目的： 用于测定小鼠血清、血浆及相关液体样本中α-防御素(α-DF)含量。 实验原理 应用双抗体夹心法测定标本中小鼠α-防御素(α-DF)水平。用纯化的小鼠α-防御素(α
α-酮戊二酸脱氢酶 α-ketoglutarate dehy-drogenase
三羧酸循环中的一种酶。是一种催化α -酮戊二酸琥珀酰 CoA反应的复合酶，需要 NAD和 CoA为辅基。 EC． 1． 2． 4、 2 α -酮戊二酸 CoA NAD 琥珀酰 CoA NADH H 此外，尚有 TPP、核酸、 FAD参予反应。整个反应机制与丙酮酸脱氢酶催化的反应极为相似。分子量约 250万。 主要为 3种单位酶组成：α -酮戊二酸酶（硫辛酰胺）（ EC1． 2． 4． 2产生琥珀二氢核糖酸，分子
甾类（化合物）17α-羟化酶 steroid 17α－hy- droxylase，s eroid 17α-monooxygeoase
甾类（化合物）17α-羟化酶 steroid 17α－hy- droxylase，s eroid 17α-monooxygeoase 存在于睾丸、肾上腺的微粒体部分。是一种在甾类分子的17α位置上引入羟基的酶。EC1、14、99、9。需要有分子态氧和NADPH存在，与P－450有关。例如将黄体酮和孕（甾）烯醇酮各自成为17α-羟基黄体酮和17α-羟基孕烯醇酮。在鼠的肾上腺中不存在此酶。
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