- 询价
- 沪震生物
- 进口/国产
- hz824Ga01
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Nuclear Factor Kappa B (NFkB)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Chicken (Gallus)
Product No. RPB824Ga01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 40.6kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Pro47~Met372 with two N-terminal Tags, His-tag and T7-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed 核因子κB(NFκB)重组蛋白below.
PYLQ IIEQPKQRGF RFRYVCEGPS HGGLPGASSE KNKKSYPQVK ICNYVGPAKV IVQLVTNGKY VHLHAHSLVG KFCEDGVCTV NAGPKDMVVG FANLGILHVT KKKVFETLET RMIDACKKGY NPGLLVHPEL GYLQAEGCGD RQLTEREREI IRQAAVQQTK EMDLSVVRLM FTAFLPDSNG GFTRRLDPVI SDAIYDSKAP NASNLKIVRM DRTAGCVTGG EEIYLLCDKV QKDDIQIRFY EEDENGGMWE GFGDFSPTDV HRQFAIVFKT PKYRDVNITK PASVFVQLRR KSDLETSEPK PFLYYPEIKD KEEVQRKRQK LM
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
核因子κB(NFκB)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验氯化镧抑制 TNF- α诱导 NF- κ B 信号通路活化 观察氯化镧 对NF-κB信号通路中关键分子的蛋白表达和磷酸化及NF-κB／p65亚基与靶基因结合能力的影响，探索氯化镧抑制NFκB活化的可能作用位点。以1×106 个／ml接种细胞于含10％胎牛血清的RMPll640液体培养基中，于5％C02 、37℃,恒温培养，随机分组如下：①TNFα组：以含TNFα20ng／ml的无血清培养基培养Hela细胞。②氯化镧+TNF-α组：分别以含氯化镧5，25，50和100
的变化，研究L6H21是否能缓解炎症反应。研究发现：1、L6H21通过抑制促分裂原活化蛋白激酶（MAPK）和核因子-κB（NFκB）信号通路，显着降低巨噬细胞中PA诱导的炎性细胞因子表达。2、L6H21还减少肾脏和心脏组织中的纤维化，以及喂食HFD的ApoE - / - 小鼠的炎症反应指数。3、这些体内作用也与L6H21对MAPK和NFκB信号传导的抑制有关。4、L6H21可能是高脂饮食诱导的心脏和肾脏损伤的潜在代理。
Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers
, the activation of the transcription factor nuclear factor κB (NFκB), induced by either phorbol ester or oxidative stress in MCF-7 cells (5 ), HaCaT keratinocytes (6 ) and HT-29 cells (unpublished results), whose growth resulted to be arrested
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