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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 库存:
大量
- 供应商:
上海善然生物科技有限公司
- 规格:
5 × 200µl
| E3581 | Gel Shift Binding 5X Buffer | 5 × 200µl | 3,222 |
| E3621 | HeLaScribe® Nuclear Extract Positive Control DNA | 300ng | 592 |
| E3641 | pGL4.37[luc2P/ARE/Hygro] Vector | 20μg | 3,570 |
| E3651 | pGL4.38[luc2P/p53 RE/Hygro] Vector | 20μg | 3,570 |
| E3661 | pGL4.39[luc2P/ATF6 RE/Hygro] Vector | 20μg | 3,570 |
| E3671 | pGL4.48[luc2P/SBE/Hygro] Vector | 20μg | 3,570 |
| E3751 | pGL4.41[luc2P/HSE/Hygro] Vector | 20μg | 3,570 |
| E3790 | rhTFIIB | 50gsu | 1,748 |
| E3971 | Reporter Lysis 5X Buffer | 30ml | 427 |
| E4001 | pGL4.42[luc2P/HRE/Hygro] Vector | 20μg | 3,570 |
| E4030 | Luciferase Assay System with Reporter Lysis Buffer | 100 assays | 839 |
| E4041 | pGL4.47[luc2P/SIE/Hygro] Vector | 20μg | 3,570 |
| E4111 | pGL4.44[luc2P/AP1 RE/Hygro] Vector | 20μg | 3,570 |
| E4121 | pGL4.43[luc2P/XRE/Hygro] Vector | 20μg | 3,570 |
| E4131 | pGL4.40[luc2P/MRE/Hygro] Vector | 20μg | 3,570 |
| E4141 | pGL4.45[luc2P/ISRE/Hygro] Vector | 20μg | 3,570 |
| E4471 | pSP-luc+NF Fusion Vector | 20µg | 749 |
| E4481 | RVprimer3 (clockwise) | 2µg | 663 |
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文献和实验an SP1 Consensus Oligo, Gel Shift Binding 5X Buffer and sufficient HeLa Nuclear Extract to perform 20 control reactions. The complete system contains five additional double-stranded oligonucleotides that represent consensus sequences to characterized
Introduction to the EMSA (Gel Shift) Technique
or agarose gel electrophoresis. Because the rate of DNA migration is shifted or retarded upon protein binding, the assay is also referred to as a gel shift or gel retardation assay. Until conception of the EMSA by Fried and Crothers3 and Garner and Revzin
Mobility-Shift DNA-Binding Assay Using Gel Electrophoresis
Transcription factors are induced in neurons to alter gene expression to adapt to brain injury caused by exposure to neurotoxins, neurodegeneration disease, or mechanical damage. These DNA-binding proteins bind to specific
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