CD3 (CD3) 抗体

CD3 (CD3) 抗体抗原
CD3 (CD3)
别名 CD3
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (KT3)
标记
其他选择 非结合性
应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), 流式细胞分析技术（FACS）
Pubmed 找到1个引用
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb118106
产品细节 CD3 (CD3) 抗体
免疫原 Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
克隆位点 KT3
亚型 IgG2a
特异性 This antibody recognises the CD3 antigen, which is expressed by mature T cells. The antibody may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
纯化方法 Affinity chromatography on Protein G
目标详细情况 CD3 (CD3) 抗体
别名 CD3
背景 T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
基因ID 12501
NCBI登录号 NP_031674
UniProt P22646
研究领域 Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
使用细节 CD3 (CD3) 抗体
应用备注 Flow Cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 1x10^6cells, stain cells at room temperature. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理 CD3 (CD3) 抗体
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4 containing 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.