L-谷氨酸/氨检测试剂盒[快速]

UV-method for the determination of L-Glutamine and Ammonia
in cell culture media, foodstuffs and other materials

Principle:
(glutaminase)
(1) L-Glutamine + H₂O → L-glutamate + NH₄⁺

(microbial glutamate dehydrogenase)
(2) NH₄⁺ + 2-Oxoglutarate + NADPH → L-glutamate + NADP⁺ + H₂O

Kit size: 50 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 15 min
Detection limit: 0.54 mg/L (L-glutamine)
0.06 mg/L (ammonia)
Application examples:
Cell culture media and cultures, dietary supplements, vegetables
and other materials (e.g. biological samples, etc.)
Method recognition: Novel method