- ¥1617
- promega
- 进口
- E3030
- 2025年12月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温
- 库存：
大量
- 供应商：
上海善然生物科技有限公司
- 规格：
40 reactions
| E3030 | Primer Extension System-AMV Reverse Transcriptase | 40 reactions | 1,617 |
| E3050 | Gel Shift Assay Core System | 100 reactions | 2,830 |
| E3091 | HeLaScribe® Nuclear Extract in vitro Transcription Grade | 40 reactions | 3,456 |
| E3092 | HeLaScribe® Nuclear Extract in vitro Transcription Grade | 160 reactions | 9,302 |
| E3110 | HeLaScribe® Nuclear Extract in vitro Transcription System | 40 reactions | 5,004 |
| E3201 | AP1 Consensus Oligonucleotide | 175pmol | 563 |
| E3202 | AP1 Consensus Oligonucleotide | 35pmol | 303 |
| E3211 | AP2 Consensus Oligonucleotide | 175pmol | 563 |
| E3212 | AP2 Consensus Oligonucleotide | 35pmol | 303 |
| E3221 | TFIID Consensus Oligonucleotide | 175pmol | 563 |
| E3222 | TFIID Consensus Oligonucleotide | 35pmol | 303 |
| E3231 | SP1 Consensus Oligonucleotide | 175pmol | 563 |
| E3232 | SP1 Consensus Oligonucleotide | 35pmol | 303 |
| E3241 | OCT1 Consensus Oligonucleotide | 175pmol | 563 |
| E3242 | OCT1 Consensus Oligonucleotide | 35pmol | 303 |
| E3281 | CREB Consensus Oligonucleotide | 175pmol | 563 |
| E3282 | CREB Consensus Oligonucleotide | 35pmol | 303 |
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文献和实验Primer Extension System - AMV Reverse Transcriptase
Primer Extension System - AMV Reverse Transcriptase The Primer Extension System is used to determine the location and level of a target 5´end of RNA. The System uses AMV Reverse Transcriptase (Cat.# M5101, M5108 and M
Add 1ml of DEPC treated water and collect eluate with purified hot primer. Reverse transcription with Superscript II from radioactive primer Take 30µg of RNA for highly expressed genes or 50µg for poorly expressed genes and adjust
A number of protocols are available for the analysis of RNA transcripts: Northern analysis (see Chapter 18 ), S1 mapping, primer extension, RNase AT1 mapping (see Chapter 20 ), and the more recent reverse transcriptase/PCR amplification
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