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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Fatty Acid Binding Protein 1, Liver (FABP1)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPB566Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 15.3kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Ser2~Ile127 (Accession # P07148) with N-terminal His-Tag
肝型脂肪酸结合蛋白(FABP1)重组蛋白
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGS-SFSGKYQLQ SQENFEAFMK AIGLPEELIQ KGKDIKGVSE IVQNGKHFKF TITAGSKVIQ NEFTVGEECE LETMTGEKVK TVVQLEGDNK LVTTFKNIKS VTELNGDIIT NTMTLGDIVF KRISKRI
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity 肝型脂肪酸结合蛋白(FABP1)重组蛋白to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验的,但是这结果是不是假阳性呢?还得做个阴性对照。阴性对照如何设置呢?考虑到整个实验体系会出现 beads、抗X抗体、诱饵蛋白 X、靶蛋白 Y(co-IP): 可能出现的假阳性来源 需要的阴性对照 抗 X 抗体结合非特异的蛋白 与 IP 一抗种属亚型相同的免疫球蛋白(比如,抗 X 抗体是 Mouse IgG,阴性对照可使用 Mouse 免疫球蛋白) beads 对蛋白的非特异结合* 同上,此外还可进行 Pre clear,IP 正式实验前排除非特异结合蛋白。如果使用琼脂
2)。 图2 硝基苯和偶氮苯的还原反应 肝微粒体中含有多种水解酶,如酯酶、酰胺酶、糖苷酶等,可分别催化酯类、酰胺类、糖苷类化合物的水解(见图3),以降低或消除其生物活性。 图3 乙酰水杨酸的水解反应 02 Ⅰ相代谢稳定性实验原理 肝药酶CYP即细胞色素P450氧化酶(CYP450),属于单加氧酶(momooxygenase),也称肝微粒体混合功能氧化酶,多位于内质网和线粒体内壁上,参与药物、致癌物、类固醇激素和脂肪酸等多种内、外源性物质代谢。肝药酶CYP氧化还原酶(POR)是所有肝
意识。 想了解 Aβ,我们还要从它的前体蛋白 APP 说起。 APP 全称淀粉样前体蛋白,英文名称为 Amyloid Precursor Protein。人类的 APP 基因位于 21 号染色体长臂,包含至少 18 个外显子。APP 分布广泛,mRNA 及其蛋白在脑、胸腺、心、肌肉、肺、肾脏、脂肪组织、肝、脾、皮肤及肠道等组织均有表达 [1]。APP 属Ⅰ型跨膜糖蛋白,分子量约 110~130kD,具有较大的膜外 (氨基端) 结构域和较小的胞质尾区 (胞内羧基端)[1]。APP 的胞外
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