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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
12个月
- 英文名:
Recombinant Glucose Transporter 1 (GLUT1)
- 库存:
100
- 供应商:
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPB185Hu01
Source Prokaryotic expression
Host E.coli
Purity > 90%
UOM 50ug
Predicted Molecular Mass 10.3kDa
Predicted isoelectric point 8.2
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
葡萄糖转运蛋白1(GLUT1)重组蛋白
Subcellular Location Cell membrane; Multi-pass membrane protein. Melanosome
Residues Met251~Glu329 (Accession # P11166) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-MMREKKVTIL ELFRSPAYRQ PILIAVVLQL SQQLSGINAV FYYSTSIFEK AGVQQPVYAT IGSGIVNTAF TVVSLFVVE
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
葡萄糖转运蛋白1(GLUT1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人葡萄糖转运蛋白 ( Glut-1 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Glut-1 单抗包被于酶标板上,标准品和样品中的 Glut-1与单抗结合,加入生物
Cancer Cell:曹雪涛院士团队揭示肿瘤相关巨噬细胞中葡萄糖代谢的增加可促进癌症转移
意义。 图片来源:Cancer Cell 主要研究内容 M2 样 TAMs 在 TME 中具有较高的葡萄糖摄取能力 为了鉴定 TAMs 中特异表达的基因,他们对其进行了单细胞转录组测序(scRNA-seq)。通过细胞聚类分析,他们区分了浸润性单核细胞、M1 样和 M2 样巨噬细胞,以及髓系祖细胞。他们注意到 Slc2a1(也被称为 Glut1)在 M2 样 TAMs 中特异性高表达,Glut1 是巨噬细胞中主要的葡萄糖转运蛋白。此外,与腹膜和脾脏巨噬细胞和外周血单核细胞相比,TAMs 在体
专家点评:Cell 重磅突破,颜宁团队和尹航团队聚焦糖转运蛋白「饿死疟原虫」
PfHT1(Plasmodium falciparum hexose transporter 1)是其主要的葡萄糖摄入蛋白,通过抑制该蛋白的转运活性,将可能有效抑制疟原虫的能量摄入从而抑制原虫的生长和增殖,但是该策略的难度在于定向抑制疟原虫的糖摄入而不影响人体细胞。在早期针对 PfHT1 作为抗疟靶点的研究中,由于缺乏该蛋白的结构信息,抑制剂的开发工作没有取得突破性进步。原清华大学生命科学学院教授颜宁领导的团队在 2014 年和 2015 年相继解析了人源葡萄糖转运蛋白 GLUT1 和 GLUT
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