- 询价
- 沪震生物
- 中国/美国
- hz048Hu01
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12个月
- 英文名：
Recombinant Inhibin Beta E (INHbE)
- 库存：
100
- 供应商：
上海沪震
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPA048Hu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 14.0kDa
Predicted isoelectric point 6.2
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Subcellular Location Secreted
Residues Thr237~Ser350 (Accession # P58166) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
抑制素βE(INHβE)重组蛋白 SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-TPTC EPATPLCCRR DHYVDFQELG WRDWILQPEG YQLNYCSGQC PPHLAGSPGI AASFHSAVFS LLKANNPWPA STSCCVPTAR RPLSLLYLDH NGNVVKTDVP DMVVEACGCS
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
抑制素βE(INHβE)重组蛋白 Ready-to-use: No need to heat, dilute or add reducing agents before use.
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文献和实验Expression of β-Adrenergic Receptors in E. coli
, such as Escherichia coli (E. coli) , that provide a reproducible and inexpensive source of receptor, combined with ease of use, low background binding, and the absence of endogenous GPCR. All these characteristics are prerequisites for the successful application
Nature 子刊：中国团队在扇贝足丝蛋白仿生材料研究领域取得重要研究进展
327 ± 32%(图 1a)，超过了绝大多数天然的生物纤维（图 1b）。通过对足丝纤维部 (图 1c) 微观结构进行观察，足丝纤维由折叠的片层组成，并且富含 β-sheet 结构 (图 1d)。基于多组学技术从足丝纤维部筛选出关键蛋白组分 Sbp5-2（图 1e）, 该蛋白具有显著的序列特点：含有多个重复模块（TRM）并且富含 Cys（图 1f）。体外重组表达该蛋白的重复模块序列成功制备了仿扇贝足丝的重组蛋白纤维（图 1 g-j）。 图 1. 扇贝足丝机械性能与结构以及重组蛋白纤维制备 体外
浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性
等人 [20] 于近期首先发现转录因子 Tcf-4 和 β-catenin 共同上调 c-Myc 基因调控因子 enhance E 的表达，然而 luciferase reporter 实验结果仅能证明 β-catenin 和转录因子 Tcf-4与enhance E 的相关性，无法提供证据表明这两种细胞因子如何作用于 enhance E 基因上。因此，他们采用了 ChIP 技术，证明前列腺癌 LnCAP 细胞中是 Tcf-4 而不是 β-catenin 直接结合到 enhance E 基因上，这就表明
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