Mast/stem Cell Growth Factor R

Mast/stem Cell Growth Factor Receptor (KIT) 抗体抗原
Mast/stem Cell Growth Factor Receptor (KIT)
别名 Bs, CD117, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, SCO1, SCO5, SOW3, Ssm, Tr-kit, W, c-KIT, C-Kit, PBT, SCFR, MGF, c-kit
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (ACK4)
标记
其他选择 非结合性
应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), 流式细胞分析技术（FACS）, 免疫萤光（IF）
Pubmed 3个引用可参考
规格 0.25 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb114259
产品细节 Mast/stem Cell Growth Factor Receptor (KIT) 抗体
免疫原 IL-3 dependent mast cells derived from WB- +/+ mice. Donor: Wistar spleen. Fusion Partner: X63.653. Ag8.
克隆位点 ACK4
亚型 IgG2a
特异性 This monoclonal antibody recognizes the receptor tyrosine kinase, c-kit. The ligand for this receptor is steel factor (stem cell factor), which exists in both soluble and membrane form. The interaction between steel factor and c-kit is essential for the development of hematopoietic, gonadal and pigment stem cells. c-kit positive cells are a subset of CD34+ hematopoietic precursor cells and it is expressed on 5-10 % of total adult bone marrow cells.
纯化方法 Protein G Chromatography.
目标详细情况 Mast/stem Cell Growth Factor Receptor (KIT) 抗体
别名 CD117 / C-Kit
背景 C-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR
基因ID 16590
NCBI登录号 NP_066922
UniProt P05532
研究领域 CD Antigens, Surface Receptors of Immune Cells, Growth Factors, Receptors, Proliferation Markers
使用细节 Mast/stem Cell Growth Factor Receptor (KIT) 抗体
应用备注 Flow Cytometry. (Reported to be useful in immunohistochemistry on frozen and polyester wax sections. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation . 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2 - 0. 1 µg of this AB per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (PE Goat anti-rat IgG (H+L)) at appropriate dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: Purified Rat IgG2aResults - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 2 µg /10e6 cellsStrains Tested: AKR, BALB/c, C3H/He, C57BL/6Positive: AKR, BALB/c, C3H/He, C57BL/6Negative: none
限制 仅限研究用
贮存及处理 Mast/stem Cell Growth Factor Receptor (KIT) 抗体
浓度 1.0 mg/mL
缓冲液 PBS containing 0.02 % Sodium Azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.