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- 2025年07月15日
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- 保存条件：
-20℃
- 克隆性：
单克隆
- 抗体名：
CD28 (CD28) 抗体 (PE)
- 规格：
0.1ml/0.2ml
CD28 (CD28)
别名 Tp44, CD28RNA
适用
其他选择 人
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (YTH913-12)
标记
其他选择 PE
CD28 (CD28) 抗体 (PE)应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 找到1个引用
规格 100 tests
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb118584
产品细节
免疫原 Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
克隆位点 YTH913-12
亚型 IgG2b
特异性 This antibody recognises the human CD28 cell surface antigen, a 44 kDa glycoprotein expressed by a subset of T lymphocytes. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.
纯化方法 Affinity chromatography on Protein G.
目标详细情况
别名 CD28
CD28 (CD28) 抗体 (PE)背景 Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
基因ID 940
NCBI登录号 NP_006130
UniProt P10747
研究领域 Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
使用细节
应用备注 Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理
溶解方式 Restore with distilled water
缓冲液 PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
储存液 Sodium azide
CD28 (CD28) 抗体 (PE)注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 This product is photosensitive and should be protected from light
储存条件 4 °C
储存方法 Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
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文献和实验CD28 及 CD28 家族其他成员 属CD28家族成员。CD28分子由两条44 kDa多肽链通过二硫键组成同源二聚体，胞膜外区有1个IgSF的V样区。 与T细胞识别、黏附和活化有关CD分子的结构 在外周血淋巴细胞中，CD28+细胞占54％一86％，包括几乎所有的CD4+T细胞和约50％的CD8+T细胞。此外，浆细胞和部分活化B细胞也可表达CD28。CD28分子的配体是B?―1(CD80)和B7―2(CD86)。CD28分子V样区中高度保守
无反应状态或死亡。 在体外联合使用 CD3 和 CD28 的抗体刺激 T 细胞，模拟 T 细胞活化的双信号作用，是进行 T 细胞激活与扩增应用最广泛的方法。除直接使用功能学抗体以外，目前磁珠法（CD3/CD28 抗体偶联磁珠）以及多聚体法（CD3/CD28 抗体偶联 Streptamer 多聚体）也是激活扩增 T 细胞比较常见的两种方法。以上说到的无论是抗体法、磁珠法、亦或者是多聚体法，归根结底都是借助于 CD3 与 CD28 抗体对 T 细胞进行刺激。 下面，我们以小鼠脾脏样本为例，分享
CD28 分子 CD28 常用单克隆抗体或代号： 9.3,4B10 主要表达细胞： Tsub, Ba,PC [T] 分子质量(kDa)和结构： gp44(IgSF)(同源二聚体) 功 能： 与CD80、CD86互为配体,提供T细胞共刺激信号 CD28 Cell adhesion molecule; co-receptor for B cell-T cell cooperation Promotes T cell activation
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