CD28 (CD28) 抗体 (PE)

CD28 (CD28) 抗体 (PE)抗原
CD28 (CD28)
别名 Tp44, CD28RNA
适用
其他选择 人
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (YTH913-12)
标记
其他选择 PE
CD28 (CD28) 抗体 (PE)应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 找到1个引用
规格 100 tests
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb118584
产品细节
免疫原 Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
克隆位点 YTH913-12
亚型 IgG2b
特异性 This antibody recognises the human CD28 cell surface antigen, a 44 kDa glycoprotein expressed by a subset of T lymphocytes. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.
纯化方法 Affinity chromatography on Protein G.
目标详细情况
别名 CD28
CD28 (CD28) 抗体 (PE)背景 Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44
基因ID 940
NCBI登录号 NP_006130
UniProt P10747
研究领域 Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis
使用细节
应用备注 Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理
溶解方式 Restore with distilled water
缓冲液 PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
储存液 Sodium azide
CD28 (CD28) 抗体 (PE)注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 This product is photosensitive and should be protected from light
储存条件 4 °C
储存方法 Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!