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- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 克隆性：
单克隆
- 抗体名：
Regulatory Factor X 4 (RFX4) (Middle Region) 抗体
- 规格：
0.1ml/0.2ml
Regulatory Factor X 4 (RFX4)
别名 NYD-SP10, 4933412G19Rik, NYD-sp10, RGD1562092
抗原表位
其他选择 Middle Region
适用
其他选择 小鼠, 大鼠, 犬, 人
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 非结合性
应用范围
其他选择 Western Blotting (WB)
Pubmed 找到1个引用
规格 100 μg
发货至 中国 (更改)
发货时间 9至12个工作日
产品编号 yb182760
产品细节 Regulatory Factor X 4 (RFX4) (Middle Region) 抗体
免疫原 Synthetic peptide directed towards the middle region of human RFX4
序列 NVDLNSITKQ TLYTMEDSRD EHRKLITQLY QEFDHLLEEQ SPIESYIEWL
预测反应 Human : 100 %, Dog : 90 %, Horse : 90 %, Rabbit : 90 %, Bovine : 80 %, Chicken : 80 %, Guinea pig : 80 %, Mouse : 80 %, Rat : 80 %
产品特性 This is a rabbit polyclonal antibody against RFX4. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Protein A purified
目标详细情况 Regulatory Factor X 4 (RFX4) (Middle Region) 抗体
别名 RFX4
背景 RFX4 is a transcription factors that contain a highly-conserved winged helix DNA binding domain. RFX4 is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. RFX4 may be a transcriptional repressor rather than a transcriptional activator.This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. This protein may be a transcriptional repressor rather than a transcriptional activator. Three transcript variants encoding different isoforms have been described for this gene.
分子量 64 kDa
基因ID 5992
NCBI登录号 NM_002920, NP_002911
UniProt Q96S80
研究领域 Chromatin and Nuclear Signaling, Transcription Factors
使用细节 Regulatory Factor X 4 (RFX4) (Middle Region) 抗体
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明
Antigen size: 563 AA
限制 仅限研究用
贮存及处理 Regulatory Factor X 4 (RFX4) (Middle Region) 抗体
状态 Lyophilized
溶解方式 Add 100 µL of distilled water.
浓度 1 mg/mL
缓冲液 PBS buffer with 2 % sucrose
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For longer periods of storage, store at -20 °C
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文献和实验A Detailed Protocol for Formaldehyde‐Assisted Isolation of Regulatory Elements (FAIRE)
displacement is a key event in the regulation of gene expression in the eukaryotic genome. This unit details an approach called Formaldehyde?Assisted Isolation of Regulatory Elements (FAIRE) for isolating nucleosome?depleted regions. FAIRE does not rely
浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性
染色质免疫沉淀（ChIP）是研究蛋白质-DNA 相互作用的一项强大技术，广泛用于多个领域的染色质相关蛋白的研究（如组蛋白及其异构体，转录因子等），特别适用于已知启动子序列或整个基因位点的组蛋白修饰分析研究。这项技术采用特定抗体来富集存在组蛋白修饰或者转录调控的 DNA 片段，通过多种下游检测技术（定量 PCR ，芯片，测序等）来检测此富集片段的 DNA 序列。 ChIP 技术自诞生之后，已成功的应用于人或动物细胞和组织 [1] 、植物组织 [2] 、酵母 [3] 以及细菌、质粒
were mapped along the promoter of CDH5 , the gene encoding VE-cadherin. Transcription factor libraries represent a useful approach for studying and modulating gene function in cells and potentially in whole organisms. Regulatory sequences
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