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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 英文名:
BioXtra, viscous liquid
- 库存:
现货
- 供应商:
上海善然生物科技有限公司
- 规格:
500ml
Frequently Asked QuestionsLive Chat andFrequently Asked Questions are available for this Product.Application非离子洗涤剂TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.CautionTWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.Preparation NoteTWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.法律信息TWEEN 为以下机构的注册商标: Croda International PLC
性质
Related CategoriesAntigen-Vaccine Preparation,Cell Culture,Core Bioreagents,Detergents,Detergents A to M,
Detergents by Applications,Diagnostic Applications,Drug Delivery-Liposomes,Electrophoresis Chromatography,Enzymology,Membrane Protein Solubilization,Non-Ionic Detergents,Research Essentials,去垢剂,生化试剂更多...description non-ionicproduct line BioXtraform viscous liquidmol wt mol wt ~1228impurities ≤0.05% Phosphorus (P)ign. residue ≤0.2%refractive index n20/D1.468(lit.)
| 91255-5MG | 杨梅苷 |
| A1153-25MG | 抑肽酶 |
| D6750-100G | 脱氧胆酸钠 |
| 88440-250ML-f | 聚乙二醇200 |
| G1251-500G | L-谷氨酸 |
| C3032-500G | 氯化铯 |
| D1626-1G | DNA(鲱鱼精) |
| C3416-5G | 羧苄 |
| I9516-500ml | 异丙醇 |
| A4737-100G | BSA |
| A1112-100G | 海藻酸钠 |
| 56485-250MG | N-羟基琥珀酰亚胺磺酸钠盐 |
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文献和实验相关专题 成功走出第一步:DNA提取 碱裂解法 大量提取质粒(500mL菌液) 1、取培养至对数生长后期(一般培养12~16小时)的含待提质粒的细菌 培养液于离心杯中,配平,4℃下4000rpm离心15min,弃上清,然后将离心管倒置于干净的约纸上使上清液全部流尽。 2、向盛有细菌 沉淀物的离心管中加入20mL冰冷的溶液Ⅰ,重悬沉淀(先加5mL溶液Ⅰ,用干净的玻璃棒搅拌均匀后,再加剩下的部分)。
【讨论】western中洗液用tween-20需要 分子级的吗
hysong 配制PBST时必须要用分子级别的吗?如果用的吐温-20是普通的化学纯的会对结果有影响吗? woxingwosu 个人觉得应该影响不大吧。 lijingxin198506 应该影响不大。 rencuiping 配制PBST时所用试剂如:NaCl 、Na2HPO4、KH2PO4、KCl均应为分析纯,所用吐温-20是普通的化学纯不会对实验结果有影响
licanming 请问大家用sigma公司的PMA时,储存液的浓度是配多少的?保存期多长?我是准备用DMSO配,我看说明书的意思是储存液浓度为20mM,但我工作液的浓度只要100nM,故很难分装和稀释。如果储存液浓度低一点,会不会对保存时间产生影响啊?我在园子里搜过,有用1mg/ml的,但我不知他是否用sigma公司的。 licanming 自己顶一下 amygdala 对于保存
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