吐温20 *Sigma货号：P7949-500ML/P794

一般描述

吐温®20是聚氧乙烯山梨醇酯，属于聚山梨醇家族
这是一种分子量为1225道尔顿的非离子洗涤剂，认为其含20个环氧yi烷单位、1个山梨醇和1个月桂酸为主要脂肪酸
环氧yi烷亚基提供了表面活性剂的亲水性，而碳氢化合物链提供疏水环境
山梨醇形成连接环氧yi烷聚合物的骨架环
[1] 吐温® 20 是一种非离子表面活性剂和去污剂，常用于生物化学和生命科学研究
其主要通途涉及膜-蛋白复合物分离过程中膜蛋白的增溶
此外，它可作为各种清洗、封闭、稀释和稳定缓冲液的成分
另外，它可用作免疫组织化学（包括蛋白质印迹和ELISA等技术）的试剂
在这些应用中，吐温® 20起到关键作用，例如防止非特异性抗体结合，zui大程度减少背景染色，确保试剂的均匀分布
表面活性剂的特征在于同时具有亲水和疏水成分，根据其在水中的电离行为可以分为离子型（阴离子、阳离子或两性）或非离子型
所述产品属于非离子型，可用于各种应用，包括乳化、分散、增溶和去污剂
此外，它也可用作膜蛋白的增溶剂
应用
Tween 20 can be used in the hybridization washing buffer after in situ hybridization. [2] It was used as a washing buffer in ELISA along with PBS (phosphate buffered saline) [3] [4] and as a washing buffer in immunostaining along with PBS (phosphate buffered saline). [5]
Tween 20 is a non-ionic detergent widely used in the field of biochemistry. It is used as an emulsifier for stable oil in water lotion. Tween 20 can be used for pre extraction of membranes to remove surrounding proteins (with a proportion of 2% used for extracting membrane-bound proteins). Tween 20 has been used as a blocking agent in membrane based immunoassays, with a commonly used concentration of 0.05%. Tween 20 can be used to lyse mammalian cells at concentrations ranging from 0.005 to 0.5%.
Non ionic detergent.
Characteristics and advantages A multifunctional surfactant used in life science and biochemistry research.
BioXtra grade products for sensitive applications. Tested trace anions and cations. Good solubilization, dispersion, and emulsifier.
Suitable for mammalian cell culture, protein purification, and heterozygous research.