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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 库存:
现货
- 供应商:
上海善然生物科技有限公司
- 规格:
500ML
TWEEN® 20
viscous liquid
Frequently Asked QuestionsLive Chat andFrequently Asked Questions are available for this Product.Application非离子洗涤剂TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.CautionTWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.Preparation NoteTWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.法律信息TWEEN 为以下机构的注册商标: Croda International PLC
性质
Related CategoriesAntigen-Vaccine Preparation,Buffers,Cell Culture,Cell Lysis Reagents,Core Bioreagents,
Detergents,Detergents A to M,Detergents by Applications,Diagnostic Applications,Drug Delivery-Liposomes,Electrophoresis Chromatography,Enzymology,Life Science Reagents for Immunohistochemistry (IHC),Membrane Protein Solubilization,Non-Ionic Detergents,Research Essentials,Signal Transduction Research Tools,去垢剂,生化试剂,细胞信号转导和神经科学,细胞生物学更多...Quality Level PREMIUMdescription non-ionicform viscous liquidmol wt mol wt ~1228composition lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)impurities ≤3.0% waterrefractive index n20/D1.468(lit.)
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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
(Sigma Cat# P-8920). - Polyoxyethylene-Sorbitan Monolaurate (Tween 20; Sigma Cat# P-1379)- Proteinase K (Boehringer Mannheim, Cat# 161 519)- Taq DNA polymerase (Promega Cat# M1861)- Tris (Boehringer Mannheim Cat# 604 205, 812 854)- Triton X
licanming 请问大家用sigma公司的PMA时,储存液的浓度是配多少的?保存期多长?我是准备用DMSO配,我看说明书的意思是储存液浓度为20mM,但我工作液的浓度只要100nM,故很难分装和稀释。如果储存液浓度低一点,会不会对保存时间产生影响啊?我在园子里搜过,有用1mg/ml的,但我不知他是否用sigma公司的。 licanming 自己顶一下 amygdala 对于保存
product code P-1379). Make up to 0.6 litres with distilled water. This is a 10x concentrate which should be diluted with distilled water as required (eg 150ml diluted with 1350ml of distilled water). * In most applications, 10mM phosphate, 0.15 M NaCl
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