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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
RNA Binding Motif Protein 10 (RBM10) (N-Term) 抗体
- 规格:
0.1ml/0.2ml
RNA Binding Motif Protein 10 (RBM10)
别名 RBM10, DXS8237E, GPATC9, GPATCH9, S1-1, TARPS, ZRANB5, E430039K10Rik
抗原表位
其他选择 N-Term
适用
其他选择 犬, 小鼠, 大鼠, 人
宿主
其他选择 兔
克隆类型
多克隆
标记
其他选择 非结合性
RNA Binding Motif Protein 10 (RBM10) (N-Term) 抗体应用范围
其他选择 免疫组织化学(IHC), Western Blotting (WB)
Pubmed 找到1个引用
规格 100 μg
发货至 中国 (更改)
发货时间 9至12个工作日
产品编号 yb182333
产品细节
免疫原 Synthetic peptide directed towards the N terminal of human RBM10
序列 MDYRSYPREY GSQEGKHDYD DSSEEQSAED SYEASPGSET QRRRRRRHRH
预测反应 Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 90 %, Pig : 90 %, Rabbit : 90 %, Rat : 90 %
产品特性 This is a rabbit polyclonal antibody against RBM10. It was validated on Western Blot and immunohistochemistry.
纯化方法 Protein A purified
目标详细情况
别名 RBM10
RNA Binding Motif Protein 10 (RBM10) (N-Term) 抗体背景 RBM10 contains RNA recognition motif found in a variety of RNA binding proteins, including various hnRNP proteins, proteins implicated in regulation of alternative splicing, and protein components of snRNPs. In vitro studies showed that the rat homolog bound to RNA homopolymers, with a preference for G and U polyribonucleotides. This gene is part of a gene cluster on chromosome Xp11.23, and its 3' end lies within 20 kb upstream of UBE1.
分子量 104 kDa
基因ID 8241
NCBI登录号 NP_005667, NM_005676
UniProt P98175
研究领域 Chromatin and Nuclear Signaling, DNA/RNA
使用细节
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明
Antigen size: 930 AA
限制 仅限研究用
贮存及处理
状态 Lyophilized
溶解方式 Add 100 µL of distilled water.
浓度 1 mg/mL
缓冲液 PBS buffer with 2 % sucrose
RNA Binding Motif Protein 10 (RBM10) (N-Term) 抗体注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For longer periods of storage, store at -20 °C
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文献和实验T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions
Ultraviolet (UV)-dependent photochemical crosslinking is a powerful approach that can be used for the identification of RNA-protein interactions. Although 8-azidoATP (8-N3 ATP) has been widely used to elucidate the ATP binding site
Using the Gibbs Motif Sampler to Find Conserved Domains in DNA and Protein Sequences
program. Keywords: Gibbs sampling; Transcription factor binding site; Sequence Alignment; Motif; DNA; Protein; Phylogentic Footprinting; Stochastic Algorithm; Markov Chain Monte?Carlo; Bayesian statistics
Active Motif ChIP 实验专家技术经验分享:如何判断 ChIP 成功与否?
抗体是否是 ChIP 级,首先都要确定抗体是否能做你的 ChIP。如果是 ChIP 级抗体,就严格遵照产品说明书做,或根据别人文章报道的方法做。如果不是 ChIP 级抗体,需要先确定是否可以做 IPIF。如果可行,那再进一步尝试是否可以用于 ChIP。比如可以尝试根据别人文章报道的方法来做。如果没有被报道过,那就只能去推测可能的结合区域,设计引物去做 qPCR 验证。如果都不能确定,那只能通过测序判断了。如果你是新手,可以同时做一个阳性对照 ChIP,比如 RNA pol II 的 ChIP(用于
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