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- 保存条件:
-20℃
- 克隆性:
单克隆
- 抗体名:
CD68 Molecule (CD68) 抗体
- 规格:
0.1ml/0.2ml
CD68 Molecule (CD68)
别名 Lamp4, Scard1, gp110, GP110, LAMP4, SCARD1
适用
其他选择 小鼠
宿主
其他选择 大鼠
克隆类型 (克隆位点)
单克隆 (FA-11)
标记
其他选择 非结合性
CD68 Molecule (CD68) 抗体应用范围
其他选择 Immunohistochemistry (Frozen Sections) (IHC (fro)), 流式细胞分析技术(FACS), 免疫萤光(IF), 免疫沉淀(IP), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), 免疫组织化学石蜡技术(IHC-P)
Pubmed 10个引用可参考
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb181837
产品细节
免疫原 Purified Concanavalin A acceptor glycoprotein from P815 cell line.
克隆位点 FA-11
亚型 IgG2a
特异性 This monoclonal FA-11 antibody does detect surface Macrosialin at low levels in resident Mouse peritoneal Macrophages which can be enhanced with thioglycollate stimulation.
交叉反应 (详细) Species reactivity (tested):Mouse.
纯化方法 Affinity Chromatography on Protein G
目标详细情况
别名 CD68
CD68 Molecule (CD68) 抗体背景 The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.Synonyms: Gp110, Macrophage marker, Macrosialin
基因ID 12514
NCBI登录号 NP_033983
UniProt P31996
研究领域 Immunology, CD Antigens, Surface Receptors of Immune Cells
使用细节
应用备注 Western Blot. Immunoprecipiation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理
浓度 1.0 mg/mL
缓冲液 PBS, pH 7.4, 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
CD68 Molecule (CD68) 抗体注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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文献和实验CD68 分子 CD68 常用单克隆抗体或代号: EBM11,Ki- M7,Ki- M6 主要表达细胞: Mac,Ptac [M] 分子质量(kDa)和结构: gp110 功 能: 参与细胞摄粒作用和溶酶体运输 CD68 Aka KP-1 May have role in macrophage phagocytic activities Specific to lysosomes, not cell
Nature Medicine 再论多重免疫荧光揭示预测性生物标志物,用于优化免疫治疗临床试验设计
同时检测(1)细胞毒性 T 细胞(CD8 和自然杀伤细胞)、Treg 细胞(CD8+、GzmA+ 、CD4+、FOXP3+/CD56+)(2) B 细胞、PC 和成纤维细胞(MUM1+、CD20+、CD27+、CD3+、COL1A1+、IgG+)(3)激活、常规 DCs 和巨噬细胞(HLA-DR、CD11c、CD1c、CD68、CD83)。对患者样本分析发现,表达 IgG 的 PC 与患者结果之间的关联表明抗肿瘤抗体的产生,这可能通过 DC 增加抗原呈递来改善 CD8 T 细胞反应。CD
15 秒止血!MIT 赵选贺团队受自然启发,研制出如此神奇的
心肌细胞的体外细胞毒性与对照组相当;通过背侧皮下移植来评估膏体的体内生物相容性,组织学评估结果表明,藤壶胶样的生物粘合剂引发了非常轻微的炎症,与 FDA 已经批准的组织粘合剂 CoSeal 相当。图片来源:Nat Biomed Eng为了进一步研究藤壶胶样的膏状物的体内生物相容性,他们对成纤维细胞的多种标记物,如 α-平滑肌肌动蛋白(αSMA)、I 型胶原以及与炎症反应相关的 T 细胞(CD3)和巨噬细胞(CD68)进行免疫荧光强度分析。结果发现,藤壶胶样的生物粘合剂在各时间点的 αSMA、I 型胶原、CD
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