CD163 Molecule (CD163) 抗体 (PE)

CD163 Molecule (CD163) 抗体 (PE)抗原
CD163 Molecule (CD163)
别名 DMBT1, CD163, CD163v2, CD163v3, M130, MM130
适用
其他选择 大鼠
宿主
其他选择 小鼠
克隆类型 (克隆位点)
单克隆 (ED2)
标记
其他选择 PE
CD163 Molecule (CD163) 抗体 (PE)应用范围
其他选择 流式细胞分析技术（FACS）
Pubmed 6个引用可参考
规格 100 tests
发货至 中国 (更改)
发货时间 29至35个工作日
产品编号 yb111837
产品细节
免疫原 Rat Spleen cell homogenate. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
克隆位点 ED2
亚型 IgG1
特异性 This Monoclonal Clone ED2 antibody recognises the Rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Clone ED2 was shown to detect approximately 50 % of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990). Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
无交叉反应 人
交叉反应 （详细） Species reactivity (tested):Rat.
纯化方法 Affinity Chromatography on Protein G
CD163 Molecule (CD163) 抗体 (PE)目标详细情况
别名 CD163
背景 CD163 is expressed by approximately 50 % of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130
基因ID 9332
NCBI登录号 NP_004235
UniProt Q86VB7
研究领域 Cardiovascular, Atherosclerosis, CD Antigens, Innate Immunity, Surface Receptors of Immune Cells
使用细节
应用备注 Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
贮存及处理
溶解方式 Restore with 1 mL distilled water.
缓冲液 PBS, 0.09 % Sodium Azide, 1 % BSA and 5 % Sucrose
储存液 Sodium azide
注意事项 Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
CD163 Molecule (CD163) 抗体 (PE)注意事项 Avoid repeated freezing and thawing. Dilute only prior to immediate use
储存条件 4 °C/-20 °C
储存方法 Store vial at 2-8 °C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.