胰酶(Sigma代理)P7545-25G

Biochem/physiol Actions

在 40°C 水中，胰酶可在 5 分钟内将不少于其质量 25 倍的马铃薯淀粉转化为可溶性糖类，在 pH 7.5 40°C 条件下，可在 60 分钟内消化不少于其质量 25 倍的酪蛋白，在 pH 9.0 37°C 条件下，每分钟每毫克来自橄榄油的胰酶可释放不少于 2 微当量的酸。

Due to the enzymatic components such as trypsin, amylase and lipase, pancreatin hydrolyzes proteins, starch and fats. Pancreatin will convert not less than 25 times its weight of potato starch into soluble carbohydrates in 5 minutes in water at 40 °C, will digest not less than 25 times its weight of casein in 60 minutes at pH7.5 at 40 °C and will release not less than 2 microequivalents of acid per min per mg pancreatin from olive oil at pH9.0 at 37 °C.

Application

Pancreatin is a mixture of several digestive enzymes produced by the exocrine cells of the porcine pancreas. It is a broad-spectrum protease composed of amylase, trypsin, lipase, ribonuclease and protease. It is used for in vitro digestibility analysis¹. Product P7545 has been used to test the sensitivities of cellulolytic bacteria inhibitors².

The enzyme has been used along with amyloglucosidase for the in vitro digestion of starch in food samples. Pancreatin from Sigma has been used for in vitro pepsin-pancreatin digestion assays while estimating of lower gut digestibility using rumen feed residues. It has also been used along with pepsin to simulate in vitro gastric and ileal digestion of raw materials in pigs.