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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 英文名:
Carbonate Bicarbonate Buffer
- 库存:
现货
- 供应商:
上海善然生物科技有限公司
- 规格:
1l
产品名称
Application
Na-carbonate (carb-bicarb) buffer for use in protein and antibody coating procedures.
Physical form
Product is a preformulated powder to be dissolved for use by diagnostic or clinical laboratories or by manufacturers of diagnostic reagents or kits. Prepare the Single Use Disposable Solution (SUDS) by dissolving the powder in 1L of purified water for a 1X working solution of 0.05 M sodium carbonate-bicarbonate buffer with pH 9.6 at 25 °C. Approximately 70:30 bicarb:carb ratio.
Suitability
Suitable for use as a coating buffer for protein-coating procedures on microtiter plates and plastic tubes for radioimmunoassay (RIA) and enzyme immunoassay (EIA) techniques
性质
| Related Categories | Application Index, Buffer Convenience Packaging, Buffer Solutions, Enzymes for Diagnostic Kit Manufacturing, Newly Upgraded Elite Products for Diagnostic Manufacturing, |
| Quality Level | GMP-IVD |
| form | powder |
| Featured Industry | Diagnostic Assay Manufacturing |
| storage temp. | 2-8°C |
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文献和实验How to Measure CFTR-Dependent Bicarbonate Transport: From Single Channels to the Intact Epithelium
Bicarbonate serves many functions in our body. It is the predominant buffer maintaining a physiological pH in the blood and within our cells. It is also essential for proper digestion of nutrients and solubilization of complex protein
Fluorescence Mounting Medium (Antifade)
Materials Needed 20ml glass scintillation vial Small stir bar Foil Glycerol 1X PBS Pipets * P-phenylenediamine ( EMD Chemicals Inc. Cat# PX0730) Carbonate-Bicarbonate Buffer
Fluorescence Mounting Medium (Antifade)
should appear almost colorless to a slight tint of yellow. If it is an intense yellow or orange color the PPD is most likely contaminated and will have background staining. pH the mounting medium to a pH of 8.0-9.0 using the Carbonate-Bicarbonate buffer
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