Cell Signaling CST 一抗 2599S Ph

Using Phospho‐Motif Antibodies to Determine Kinase Substrates

in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides

运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白

using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative

【求助】关于gsk3-beta功能的疑问

=result&resnum=1#v=onepage&q=gsk%20tyrosine%20phosphorylation%20and%20activity&f=false By the way, based on my own experience, most of antibodies from Santa Cruz are NOT reliable at all. I strongly recommend Cell Signaling Technology (CST