L-Asparaginase

Introduction L-Asparaginase is an enzyme that depletes L-Asparagine "an important nutrient for cancer cel" resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E.coli.,which can inhibit the growth of malignant cel. It is used mainly for the induction of remission in acute lymphoblastic leukaemia. Because of the lymph node origin of malignant B cel in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease.

The rationale behind asparaginase is that it takes advantage of the fact that ALL celare unable to synthesize the non-essential amino acidasparaginewhereas normal cel are able to make their own asparagine. These leukemic cel depend on circulating asparagine. Asparaginase however catalyzes the conversion of L-asparagine to aspartic acidand ammonia. This deprives the leukemic cell of circulating asparagine.

L-AsparaginaseDescription L-Asparaginase produced from E.Coli containing 303 amino acids and having a molecular mass of 31731 Dalton.

Source Escherichia Coli.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation The enzyme was lyophilized with no additives.

Purity Greater than 96.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by S-PAGE.

Stability Two years when stored at -20℃, 2 weeks at 4℃.

L-AsparaginaseUnit Defenition One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.

Activity One IU of L- Asparaginase is defined as that amount of enzyme required to generate 1 µmol of ammonia per minute at pH 7.3 and 37℃.

Specific Activity 225 IU/mg.