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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
钰博生物
- 细胞类型:
复苏
- 组织来源:
见说明书
- 物种来源:
人、大鼠、小鼠、、、、
- 免疫类型:
请咨询销售人员
- 细胞形态:
上皮样;多角形
- 是否是肿瘤细胞:
见说明书
- 器官来源:
见说明书
- 运输方式:
干冰运输or活细胞运输
- 年限:
见说明书
- 生长状态:
贴壁生长
Recombinant E.Coli Cytoplasmic L-asparaginase I
Synonyms L-asparaginase 1, L-asparaginase I, L-ASNase I, L-asparagine amidohydrolase I, ansA, b1767, JW1756.
Introduction AnsA is a cytoplasmic asparaginase from E.coli involved in intracellular asparagine utilization. E.coli has 2 L-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type L asparaginase) is constitutively expressed and is obligatory for the growth of the bacteria on asparagine as the sole nitrogen source.
Recombinant E.Coli Cytoplasmic L-asparaginase I Description ANSA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 358 amino acids (1-338 a.a.) and having a molecular mass of 39.3kDa.
ANSA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source Escherichia Coli.
Physical Appearance Sterile Filtered colorless solution.
Recombinant E.Coli Cytoplasmic L-asparaginase I Formulation The ANSA solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 2mM DTT.
Stability ANSA should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD.
Recombinant E.Coli Cytoplasmic L-asparaginase I Usage CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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):单倍体基因组中DNA 的总量。 CAAT box (CAAT 盒):真核生物转录单位起始点上游的保守序列,被一组转录因子识别。 Cap (帽):是真核生物mRNA 5‘端的结构,在转录后通过末端5‘GTP的磷酸基团和mRNA的末端碱基而引入。增加的G(有时是其它碱基)是甲基化的,产生了MeG5‘pppNp… 的结构。 CAP(CRP):由cAMP 激活的正调控蛋白质。对RNA 聚合酶起始E.coli 中一些操纵子(分解代谢——敏感)是必须的。 Capsid (衣壳):是病毒微粒外部
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Recombinant E.Coli Cytoplasmic L-asparaginase I
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