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文献和实验cells, count and dilute to 4 x 105 cells/ml in cell culture media. 4. Dilute the molecules to be tested to required concentration in cell culture media (Make 2x of the final required concentration). Filter this and serially dilute 1:10, 1:100, 1:1000
He Tube Mating with MSCV recipient
Chemicals needed: Glucose L-(+)-Arabinose Sigma Cat # A3256 L-Rhamnose Sigma cat # R3875 Cb (carbenicillin) Sigma cat # 1389 DL-p-Chlorophenylalanine Sigma cat # C6506 Media Recipes: YEG recombination plates: 1 liter Milli Q water
Metabolite Quantitation of Media Post-AA Labeling
tube and vortex again. Do not transfer any of the pellet, even if it is necessary to sacrifice some of the sample. 6) Add 2 ml of chloroform to break phases and vortex vigorously. 7) Add 2ml of water and again vortex vigorously. 8) Spin
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