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文献和实验SDS-PAGE Western Blotting Protocols
). Part II – SDS-Polyacrylamide Gel Electrophoresis 1. For each SDS-PAGE gel, sandwich one small and one large glass plate,separated by spacers (smeared with silicone lubricant) and an alignment card. Optional: The inside surface of the small
The ribonuclease protection assay (RPA)
plate, and absorb the gel to filter paper. Cover the gel with Saran wrap and layer between two additional pieces of filter paper. Place in the gel dryer vacuum for ~ 1 hour at 80°C. Place the dried gel on film (Kodak X-AR) in a cassette
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
(Vector Laboratories PK 6100) (optional, see Step 30) Wash solution 1 Wash solution 2 Equipment Aluminum foil Diamond pen Eye protection (see Step 21) Filter paper (e.g., Whatman 3MM) Humid chamber for slide
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