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文献和实验Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
/ ml = 4 × 106 cells / ml). cells / ml × original volume = Total cells (e.g. 40 million cells in 10 ml) cell viability (%): total viable cells (unstained) / total cells (stained and unstained) × 100 (e.g. 20 stained cells per quadrant: 50% viability)
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
Adult cattle serum as supplemental nutrient in lymphocyte cultures for chromosomes studies
containing various ingredients to induce division, support enhanced growth, and increase the number and quantity of cells. In this pursuit different kinds of synthetic media have been developed and are commercially available. These media contain amino acids
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