GST Tag Mouse Monoclonal Antib

有适合免疫沉淀IP的GST抗体吗？

应用。用GST融合表达系统表达外源基因时，需要对融合表达的产物进行检测和纯化，这里面就包括了GST标签抗体的应用，如果选择一个最具性价比的GST抗体显得尤为重要。首先需要考虑抗体的来源，比如小鼠源的单克隆抗体还是兔源的多克隆抗体，一般来说，单抗的特异性、稳定性以及效价都要优于多克隆抗体。EarthOx的GST标签抗体――Anti-GST Tag Monoclonal Antibody [GST.B6]（货号#E022040）是来源于小鼠的单克隆抗体，克隆号为GST.B6，免疫原为GST多肽 。

GST-fusion protein injection into rabbits

for antibody production Depending on the antiginicity of your fusion protein, a range of 20-100 mg of fusion protein is required for first injection. NOTE: When using GST-fused to small peptide (10-20 aa) use ~250-500 mg of protein/injection.

Isolation of Human Monoclonal Antibodies Using Guided Selection with Mouse Monoclonal Antibodies

Repertoires of antibody (Ab) V genes derived from nonimmunized human donors (1 ) or made synthetically (2 3 ) have been cloned for display on filamentous bacteriophage as either scFvs or Fabs fused to the minor phage coat protein (pIII