Recombinant Human DNA Topoisom

Purification of DNA Topoisomerase I from Human Placenta

DNA topoisomerase I (topo I) has been firmly established as the molecular target of the camptothecin group of anticancer drugs. These drugs include camptothecin, topotecan, 9-amino-camptothecin, and irinotecan

Purification of Baculovirus-Expressed Human DNA Topoisomerase I

DNA topoisomerase I (topo I) can be isolated from cultured human cells in quantities that are more than sufficient for investigations into the ability of topo I to relax supercoiled DNA (250 μg/109 cells) (1 ,2 ). However, the production

Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA

of nicked and supercoiled forms of the circular DNA to compete for the binding of a 3 H-labeled nicked DNA to the topoisomerase where the enzyme–DNA complexes are quantitated by the retention of the labeled DNA on a nitrocellulose membrane.