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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase (ST8SIA4)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase (ST8SIA4)品名简称:
Recombinant Human ST8SIA4 protein货号:
CSB-EP821630HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
ST8SIA4Uniprot No.:
Q92187别名:
Alpha-2,8-sialyltransferase 8DPolysialyltransferase-1Sialyltransferase 8D ;SIAT8-DSialyltransferase St8Sia IV ;ST8SiaIV种属:
Homo sapiens (Human)蛋白长度:
Full Length of Isoform 2来源:
E.coli分子量:
32.8 kDa表达区域:
21-168aa氨基酸序列:
KTKEIARTEEHQETQLIGDGELSLSRSLVNSSDKIIRKAGSSIFQHNVEGWKINSSLVLEIRKNILRFLDAERDVSVVKSSFKPGDVIHYVLDRRRTLNISHDLHSLLPEVSPMKNRRFKTCAVVGNSGILLDSECGKEIDSHNFVIR蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the bryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.功能2:
Catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid (PSA), which is present on the embryonic neural cell adhesion molecule (N-CAM), necessary for plasticity of neural cells.文献:
Expression cloning of a human polysialyltransferase that forms the polysialylated neural cell adhesion molecule present in embryonic brain.Nakayama J., Fukuda M.N., Fredette B., Ranscht B., Fukuda M.Proc. Natl. Acad. Sci. U.S.A. 92:7031-7035(1995)研究领域:
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文献和实验Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0614, USA Biochem Biophys Res Commun 224: 103-7 (1996)CMP-NeuAc:GM1 alpha 2,3-sialyltransferase (ST-IV) was purified to homogeneity from rat brain. Microsequencing
。 细菌来源细胞因子 上世纪70年代出现的基因工程重组技术,使得使用原核细菌宿主,如大肠杆菌进行天然蛋白的重组表达和生产风靡一时,因其速度快、生产成本低、产率高等优点,广泛用于包括细胞因子在内的多种重组蛋白表达的科研和生物制药领域。但随之而来,使用低等细菌进行蛋白表达的技术弊端也日益显现:低等原核细菌宿主表达的细胞因子,其结构和天然细胞因子蛋白有较大的差异。由于缺乏高等动物细胞中特有的各种细胞器,细菌表达的蛋白肽链无法完成翻译后修饰,缺乏必要的糖基化和磷酸化,二硫键配对完全随机;细菌
Introduction of human 14-3-3 proteins
Biophysics,Medical College of Virginia,Virginia Commonwealth University,Richmond 23298-0614,USABiochem Biophys Res Commun 224: 103-7 (1996)CMP-NeuAc:GM1 alpha 2,3-sialyltransferase (ST-IV)was purified to homogeneity from rat brain.Microsequencing
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