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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human HLA-B protein (HLA-B), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human HLA-B protein (HLA-B), partial品名简称:
Recombinant Human HLA-B protein, partial货号:
CSB-RP150894h规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
HLA-BUniprot No.:
Q96DW9别名:
/种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
36.8 kDa表达区域:
25-308aa氨基酸序列:
GSHSMRYFYTAMSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQICKTNTQTYRESLRNLRGYYNQSEAGSHTLQRMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLSSWTAADTAAQITQRKWEAAREAEQLRAYLEGLCVEWLRRYLENGKETLQRADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTIPI蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in the presentation of foreign antigens to the immune syst.SAAS annotation功能2:
/文献:
"Study on polymorphism of MICA exon 5 microsatellite in Shanghai Han population."Guo Z., Xu L., Fan L., Ge Y., Yao F., Yang J.Zhonghua Yi Xue Yi Chuan Xue Za Zhi 17:332-335(2000)研究领域:
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文献和实验症状,体格检查和相关实验室指标无异常。ERA 患儿依据 ILAR 相关标准明确诊断。由于研究人群的特殊性,临床上完全健康的体检儿童较少,我们将一些先心病、腺样体肥大等对 HLA-B27 表达影响小的患儿纳入到正常组。 流式细胞仪及配套 HLA-B27 试剂盒、溶血素均为同一厂家提供。以 CD3 - 藻红蛋白(phycoerythrin,PE)标记的 T 淋巴细胞设门,检测门内细胞 HLA-B27 表达的平均荧光强度。正态分布的检测数据以(x±S)表示,组间比较采用单因素方差分析;偏态分布的检测数据以中位
)与b2微球蛋白(轻链)形成二聚体分子(HLA-I类分子)。该分子分布于有核细胞的表面,具有特定结构的内源性抗原的提呈功能。抗原在胞浆内经酶降解成小的肽片段,在内质网与HLA-B*27编码产生的HLA-I类分子专一性结合成复合物,然后转送到细胞膜表面,供CD8+ T细胞识别,从而激活该细胞。此外,早期T细胞在胸腺中发育为成熟T细胞的过程中,也必须与表达HLA-Ⅰ类分子的胸腺上皮细胞接触才能分化成CD8+ T细胞。 图一:HLA-B27分子及其编码基因示意图(摘自:医学免疫学/陈慰峰主编,第三
由于AS发病隐匿,早期症状不明显,且变化多端,一些早期症状如腰骶、下背痛及晨僵不易引起患者注意;加上与类风湿关节炎等多种疾病的症状相似,临床上对早期患者极易造成误诊和漏诊,因此,寻找一种可靠的实验室检查项目即显得十分重要。[2] 70年代初期,发现白细胞抗原(human leukocyte antigen,HLA) HLA-B27抗原与AS密切相关,此后,HLA-B27在AS诊断中的作用日益受到重视,已成为临床上AS的重要辅助诊断方法。[3,4] HLA-B27基因是人类HLA-I类分子B位点
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