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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Calmodulin-like protein 5 (CALML5)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Calmodulin-like protein 5 (CALML5)品名简称:
Recombinant Human CALML5 protein货号:
CSB-EP973713HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
CALML5Uniprot No.:
Q9NZT1别名:
Calmodulin-like skin protein种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
31.8 kDa表达区域:
2-146aa氨基酸序列:
AGELTPEEEAQYKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDSDGDGEISFQEFLTAAKKARAGLEDLQVAFRAFDQDGDGHITVDELRRAMAGLGQPLPQEELDAMIREADVDQDGRVNYEEFARMLAQE蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Binds calcium. May be involved in terminal differentiation of keratinocytes.功能2:
Binds calcium. May be involved in terminal differentiation of keratinocytes.文献:
Identification and cloning of a new calmodulin-like protein from human epidermis.Mehul B., Bernard D., Simonetti L., Bernard M.A., Schmidt R.J. Biol. Chem. 275:12841-12847(2000)研究领域:
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文献和实验[资源][原创] 蛋白标签纯化技术——CBP(钙调蛋白结合肽)
基于CBP标签技术的蛋白质表达与纯化系统 (用于高水平蛋白质表达和单步骤纯化)Protein Expression and Purification System是一种简单、温和、有效的方法,包括一个含有 calmodulin-binding peptide tag 钙调蛋白结合肽标签的pCAL 表达载体和一个用于分离的钙激酶亲合树脂,可快速敏感的捕捉标签融合蛋白。T7 RNA polymerase-based pCAL 载体能够快速克隆钙调蛋白结合肽标签融合蛋白。CBP标记的蛋白在低浓度钙
(old tuberculin,OT)是柯赫(R.Koch)首先发明的。而纯蛋白衍生物(purified protein derivative,PPD)则是由塞伯尔(Seibert)首先制备的。结核菌素试验方法很多,常用的是芒图(Mantoux)氏法,即将OT或PPD用无菌生理盐水稀释成不同浓度,取0.1毫升注射于前臂掌侧皮内,48~72小时后检查反应情况,应注意局部有无硬节,不可单独以红晕为标准。若注射部位有针眼大的红点或稍有红肿,硬节直径小于0.5厘米,则为阴性反应。若注射部位硬节直径超过
and printed onto microscope slides. These protein microarrayswere used to identify novel calmodulin- and phospholipid-interacting proteins (180). These reports indicate that protein arrays hold great promise for the global analysis of protein-protein and
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