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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Calmodulin-like protein 5 (CALML5)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Calmodulin-like protein 5 (CALML5)品名简称:
Recombinant Human CALML5 protein货号:
CSB-EP973713HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
CALML5Uniprot No.:
Q9NZT1别名:
Calmodulin-like skin protein种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
31.8 kDa表达区域:
2-146aa氨基酸序列:
AGELTPEEEAQYKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDSDGDGEISFQEFLTAAKKARAGLEDLQVAFRAFDQDGDGHITVDELRRAMAGLGQPLPQEELDAMIREADVDQDGRVNYEEFARMLAQE蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Binds calcium. May be involved in terminal differentiation of keratinocytes.功能2:
Binds calcium. May be involved in terminal differentiation of keratinocytes.文献:
Identification and cloning of a new calmodulin-like protein from human epidermis.Mehul B., Bernard D., Simonetti L., Bernard M.A., Schmidt R.J. Biol. Chem. 275:12841-12847(2000)研究领域:
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文献和实验[资源][原创] 蛋白标签纯化技术——CBP(钙调蛋白结合肽)
基于CBP标签技术的蛋白质表达与纯化系统 (用于高水平蛋白质表达和单步骤纯化)Protein Expression and Purification System是一种简单、温和、有效的方法,包括一个含有 calmodulin-binding peptide tag 钙调蛋白结合肽标签的pCAL 表达载体和一个用于分离的钙激酶亲合树脂,可快速敏感的捕捉标签融合蛋白。T7 RNA polymerase-based pCAL 载体能够快速克隆钙调蛋白结合肽标签融合蛋白。CBP标记的蛋白在低浓度钙
的靶点分布呈现出明显集中的特点,主要集中在少数几个关键的激酶家族上[19]。 表3. 获批激酶抑制剂的靶点分布[19]。 总的来说,已获批激酶抑制剂的靶点分布高度集中于驱动癌症和自身免疫性疾病的关键信号通路上,尤其是酪氨酸激酶家族。 MCE 相关产品 一、主要激酶家族的重组蛋白:含常见突变体如 EGFR L858R/T790M/C797S(www
(old tuberculin,OT)是柯赫(R.Koch)首先发明的。而纯蛋白衍生物(purified protein derivative,PPD)则是由塞伯尔(Seibert)首先制备的。结核菌素试验方法很多,常用的是芒图(Mantoux)氏法,即将OT或PPD用无菌生理盐水稀释成不同浓度,取0.1毫升注射于前臂掌侧皮内,48~72小时后检查反应情况,应注意局部有无硬节,不可单独以红晕为标准。若注射部位有针眼大的红点或稍有红肿,硬节直径小于0.5厘米,则为阴性反应。若注射部位硬节直径超过
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