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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store active CHK1 at -80ºC, ATP Solution at -20ºC and all other kit components at 4ºC. Avoid multiple freeze/thaw cycles.
- 英文名:
96-well Checkpoint Kinase Activity Assay Kit
- 库存:
大量
- 供应商:
欣博盛
- 规格:
96 assays
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文献和实验Mammalian CHK1 is a Ser/Thr effector kinase that plays critical roles in the DNA damage-activated cell cycle checkpoint signaling pathway downstream of ATR (ATM and Rad3-related protein kinase). This chapter is focused on describing an assay
An In Vitro Assay for the Kinase Activity of mTOR Complex 2
with DEPTOR and positively by the TSC1–TSC2 protein complex. To study the regulation of mTORC2 activity, it is a common practice to examine the phosphorylation of its substrates in vivo and verify the findings with an in vitro assay of kinase activity
MAP Kinase Regulation of the Mitotic Spindle Checkpoint
signaling, causes an increase in MAPK activity that inhibits Aurora B kinase activity. By monitoring mitotic index and transit time from nuclear envelope breakdown to anaphase, we demonstrated that RKIP depletion leads to a defective spindle checkpoint
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