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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 英文名:
GFP-Tag Rabbit monoclonal antibody
- 供应商:
上海博尔森生物科技有限公司
- 保存条件:
-20℃
- 规格:
100μL
GFP-Tag Rabbit monoclonal antibody
Product Name Anti-GFP (Green fluorescent protein) polyclonal antibody
Immunogen: Recombinant GFP expressed in E.coli
Purification: antigen affinity purification
Tested applications: WB IP IHC IF. Recommended dilutions: WB: 1:2000-5000 IHC: 1:200 IP 1:200 IF: 1:200
Optimal dilutions/concentrations should be determined by the end user. Form: Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: GFP antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. Reactivity: ALL

PET-28a GFP recombinant protein expressed in E.coli Primary
antibody Anti-GFP PAB (GFP 多抗) lane 1:1:5000 lane 2 1:10000
Secondary antibody Goat-anti Rabbit lgG (H&L) HRP (AB2103)
1:10000
GFP-Tag Rabbit monoclonal antibody
Product Name Anti-GFP (Green fluorescent protein) polyclonal antibody
Immunogen: Recombinant GFP expressed in E.coli
Purification: antigen affinity purification
Tested applications: WB IP IHC IF. Recommended dilutions: WB: 1:2000-5000 IHC: 1:200 IP 1:200 IF: 1:200
Optimal dilutions/concentrations should be determined by the end user. Form: Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: GFP antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. Reactivity: ALL

PET-28a GFP recombinant protein expressed in E.coli Primary
antibody Anti-GFP PAB (GFP 多抗) lane 1:1:5000 lane 2 1:10000
Secondary antibody Goat-anti Rabbit lgG (H&L) HRP (AB2103)
1:10000
本产品只适用于科研,不能用于临床诊断。严禁用于临床医疗及其他非科研用途!
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文献和实验【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
对照,2道为1:1000倍稀释我们先看一下EarthOx的单克隆GFP抗体——Anti-GFP Tag Monoclonal Antibody [GF28R](货号#E022030),其免疫原是KLH偶联的水母GFP蛋白的N末端多肽,由于EGFP、YFP、EYFP、CFP得N末端序列与GFP几乎完全一致,因此从免疫原上来说,GFP与EGFP、YFP、EYFP、CFP没有区别。更重要的是,EarthOx对于该单克隆抗体还进行了明确的验证试验,试验也表明该Anti-GFP Tag Monoclonal
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
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