- ￥1700 - 4000
- GeneTex
- 美国
- GTX112964
- 2025年07月16日
- WB, ICC/IF, IHC-P, IP
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX112964
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP
- 浓度：
0.82 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HSP27
- 抗体英文名：
HSP27 antibody
- 抗体名：
HSP27 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Wild-type (WT) and HSP27 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX112964) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX112964) diluted at 1:5000.
HSP27 antibody immunoprecipitates HSP27 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HSP27 protein by 2.5 μg of HSP27 (GTX112964) 15% SDS-PAGE The immunoprecipitated HSP27 protein was detected by HSP27 antibody (GTX112964) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using HSP27(GTX112964) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
HSP27 antibody detects HSP27 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: HSP27 protein stained by HSP27 antibody (GTX112964) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
12% SDS PAGE
GTX112964 diluted at 1:1000
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文献和实验Delphine B. et al., JND 2016 Preprint()
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