- ￥1700 - 4000
- GeneTex
- 美国
- GTX101145
- 2025年07月16日
- WB, ICC/IF, IHC-P, FACS, IP
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX101145
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, FACS, IP
- 浓度：
0.45 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
HSP27
- 抗体英文名：
HSP27 antibody
- 抗体名：
HSP27 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Non-transfected (–) and transfected (+) HT-29 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Wild-type (WT) and HSP27 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of HSP27 protein from HeLa whole cell extracts using 5 μg of HSP27 antibody (GTX101145).
Western blot analysis was performed using HSP27 antibody (GTX101145).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Sample (50 μg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX101145 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody (GTX101145) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HSP27 antibody detects HSP27 protein at cytoplasm in human lung papillary carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung papillary carcinoma.
HSP27 antibody (GTX101145) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
HSP27 antibody detects HSP27 protein at cytoplasm and cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: HSP27 protein stained by HSP27 antibody (GTX101145) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
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文献和实验Chou HC et al., Arch Biochem Biophys 2014 (PMID:24384558)
Cui X et al., Dis Model Mech 2022 (PMID:35099007)
Xu YM et al., Toxicol Res (Camb) 2019 (PMID:32922740)
Cheung CHY et al., J Biomed Sci 2020 (PMID:32576196)
Xu YM et al., Oncotarget 2016 (PMID:26716417)
Huang HJ et al., Mol Biosyst 2014 (PMID:25259860)
Lin ST et al., J Proteomics 2012 (PMID:22889595)
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