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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4ºC
- 克隆性:
多克隆
- 抗体英文名:
EAAC1 Antibody: ATTO 700
- 抗体名:
EAAC1 Antibody: ATTO 700
- 规格:
100 µg
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文献和实验The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
by differential fluorometry. Because a critical ingredient for the target enrichment is an immobilization matrix made out of hundreds of thousands of microscopic, antibody-coated beads, we have termed this detection method an a ssay with a l arge i mmuno-s orbent
. 4. When finished, immerse membrane in Blocking buffer and block overnight. H. Antibodies and detection 1. Incubate with primary antibody diluted in Blocking buffer for 2 hours at room temp. 2. Wash 3 x 10 min with PBST. 1 x10min secondary washing
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