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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Fusion protein amino acids 77-200 of human PSD95/SAP90
- 亚型:
IgG2a
- 保存条件:
4°C
- 克隆性:
Monoclonal
- 标记物:
ATTO 488
- 适应物种:
Mouse, Rat, Human
- 宿主:
Mouse
- 应用范围:
WB, IHC, IF, IP, Immuno-gold EM
- 浓度:
1mg/mL
- 靶点:
S28-43
- 抗体英文名:
PSD95 Antibody: ATTO 488
- 规格:
100μg
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文献和实验Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr(图)
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
感;良好的稳定性和活性染料的标记率。Alexa Fluor 488在较宽的PH值范围内保持稳定(PH4~10);FITC激发波长488nm,最大发射波长525nm;缺点荧光强度易受PH值影响,PH值降低时其荧光强度减弱。橙红色(543-555nm处激发) CF 543、AlexaFluor 546、ATTO550, Cy 3,DyLight 549, Rhodamine (TRITC) 匹配543nm的橙色荧光染料;CF ? 543 荧光条带明亮,耐光,水溶性好,确保了CF 543染料与抗体的耦联物
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