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文献和实验Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
9 (TG30) monoclonal antibody (Millipore, cat. no. MAB4427) Mouse anti‐GCTM2 monoclonal antibody (0.4 mg/ml; kind gift from Martin Pera)—a commercially available alternative, which detects
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
) Hemoglobin marker: 1 g lyophilized human hemoglobin (Sigma) resuspended in 600 µl PBS Goat anti–human C4 (DiaSorin)
Dual- and Triple-Color Fluorospot
for the other cytokine (Fig. 1). As a second step in the detection, streptavidin conjugated to a red fluorophore (Cy3) and anti-FITC antibodies labeled with a green fluorophore, respectively, are used (10, 11). The resulting spots are subsequently analyzed using
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