Merck Millipore Anti-phospho-P

v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –

Using Phospho‐Motif Antibodies to Determine Kinase Substrates

in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides

Determining In Vivo Phosphorylation Sites Using Mass Spectrometry

Basic Protocol 2: Global Phosphorylation Analysis (Ser/Thr/Tyr) Alternate Protocol 2: SDS‐PAGE Protein Fractionation of Whole‐Cell Extracts Basic Protocol 3: Phosphotyrosine (pTyr) Site Identification