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南京赛泓瑞
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5 UG RHUMAN
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
are performed by inducing in vitro and in vivo apoptosis and then visualized the phenomenon with anti-DNA mAb. In vitro experiments are performed by treating human lymphocytes with 2-deoxyribose and rat thymocytes with dexamethasone 21-phosphate. In vivo
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
al. 2006b ). MATERIALS Reagents Anti-CD24 monoclonal antibody (mAb) (J11d clone) (for Protocol 3) Use either culture supernatant that contains anti-CD24 mAb or purified anti-CD24 mAb (see Step 55). BDPharmLyse (red blood cell lysing
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