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文献和实验Apoptosis Measurement by Annexin V Staining
assay. Recently, PARP cleavage (7 ) and annexin V (8 –11 ) have claimed more specificity. PARP cleavage occurs early in the response as a result of the activity of caspase-3 and correlates well with chromatin condensation (12 ). PARP cleavage
MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol
from DiOC1(5). The example below is a protocol for labeling with an annexin V–Alexa Fluor® 488 conjugate and propidium iodide. 1) After step 3 (above), wash cells once by adding 2 mL of warm phosphate-buffered saline or other buffer to each tube
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
-catenin and GSK3 beta. Curr. Biol. 8, 573–581. 17. Itoh, K., Krupnik, V. E., and Sokol, S. Y. (1998) Axis determination in Xenopus involves biochemical interactions of axin, glycogen synthase kinase 3 and beta-catenin. Curr. Biol. 8, 591–594. 18. Laemmli, U
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