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文献和实验Phosphatasing with Shrimp Alkaline Phosphatase (S.A.P.)
Phosphatasing with Shrimp Alkaline Phosphatase (S.A.P.) Use to prevent linearized vector plasmid from recircularizing. The phosphatase from shrimp is easier to get rid of than is CIP, because SAP is heat labile.
in solution c. for 5-15 minutes at room temperature. Wash, counterstain in haematoxylin, wash. Mount in phosphate buffered glycerine jelly RESULTS Alkaline phosphatase activity - Red Nuclei - Blue
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity
Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity 1. Obtain embryo samples, tube of AP substrate buffer and tube of phosphate buffered saline ( PBS ) for each group. Allow embryos
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