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南京赛泓瑞
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100 UG
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文献和实验An Enzymatic Microplate Assay for Testing P-Glycoprotein Substrates and Inhibitors
it possible to detect specific interactions between drugs and P-gp. They took into account the existence of multiple binding sites on P-gp to finalize an optimized strategy that involves the assay of the P-gp ATPase stimulated by three reference substrates
anchor. First, control studies are detailed to test for nonspecific liposome immobilization (fluorescence assay; see Subheading 3.2), and to ensure that liposomes remain intact on the microplate surface (dye leakage assay; see Subheading 3.3). Finally
The biotin–avidin microplate assay is a sensitive method to measure methylation of biotinylated oligonucleotide substrates by DNA methyltransferases (MTases). The methylation reaction is carried out in solution using [methyl-3 H]-AdoMet
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