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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
西宝生物
- 库存:
1KG
- 保存条件:
室温
- 英文名:
TRIS, ULTRA PURE , 99.9%
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文献和实验培养细胞RNA提取--RNA Isolation Protocol-Cultured Cell Samples
Required Reagents: Chloroform Isopropanol 75% Ethanol (cold) Monophasic Isolation Reagent RNA Precipitation Solution 1.2M NaCl 0.8M Disodium Citrate DNase (RNase-free) Ultra-pure water Liquid Nitrogen Solutions
Replication timing by density transfer
. Filter-sterilize; do not autoclave. The refractive index of the solution should be 1.3393-1.3395. CsCl : from Gallard-Schlesinger Industries, Carle Place, NY 11514-1731. It's 99.9% pure "Special Biochemical Grade", Catalog # 612501, $150/kilogram.
Stripping for reprobing western blots
pH 6.8 0.5 M 67.5 ml ultra pure water Add 0.8 ml ß-mercaptoethanol under the fumehood. 实验步骤 1. Membrane incubation (1) Use a volume that will cover the membrane. Incubate at room temperature for 5-10
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