- 询价
- WAKO
- 590-33585
- 进口
- 2025年10月14日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
室温
- 英文名：
BEEF EXTRACT
- 供应商：
西宝生物
- 库存：
500G
- 规格：
100 UL BIOSOURCE (TM)
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Preparation of nuclear extract and cytoplasmic extract
PMSFBuffer D (Dialysis Buffer): For 2 L20 mM HEPES,pH 7.9 1 M HEPES 7.9 40 ml20 % Glycerol Glycerol 400 ml100 mM KCl3 M KCl66.67 ml0.2 mM EDTA 0.5 M EDTA 800 µl0.5 mM DTT 1M DTT 1 ml0.2 mM PMSF 0.2 M PMSF 2 mlSplicing Extract Preparation1.Resuspend cell
us to succesfully immunodeplete and characterize three different proteins: a) During extract prep, after washing out cysteine with XB, wash eggs 2-3x with CSF-XB (no PIs) before washing with CSF-XB + PIs and then process as usual. b) Crush the eggs at 10
+ PIs. Add 150 ul of clarified extract to each tube. Rotate for 1 hr at 4 deg.C ensuring that beads are mixing well. Pellet and transfer supe to a different tube. Aliquot and freeze 20 ul aliquots in green tubes (XKCM1 deplete
技术资料暂无技术资料 索取技术资料
